The regulatory mechanism of the yeast osmoresponse under different glucose concentrations
Wenting Shen,
Ziqing Gao,
Kaiyue Chen,
Alusi Zhao,
Qi Ouyang,
Chunxiong Luo
Affiliations
Wenting Shen
The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing, China; Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China
Ziqing Gao
Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China
Kaiyue Chen
The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing, China; Wenzhou Institute University of Chinese Academy of Sciences, Wenzhou, Zhejiang, China; Oujiang Laboratory, Wenzhou, Zhejiang, China
Alusi Zhao
The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing, China
Qi Ouyang
The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing, China; Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China; Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China
Chunxiong Luo
The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing, China; Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China; Wenzhou Institute University of Chinese Academy of Sciences, Wenzhou, Zhejiang, China; Oujiang Laboratory, Wenzhou, Zhejiang, China; Corresponding author
Summary: Cells constantly respond to environmental changes by modulating gene expression programs. These responses may demand substantial costs and, thus, affect cell growth. Understanding the regulation of these processes represents a key question in biology and biotechnology. Here, we studied the responses to osmotic stress in glucose-limited environments. By analyzing seventeen osmotic stress-induced genes and stress-activated protein kinase Hog1, we found that cells exhibited stronger osmotic gene expression response and larger integral of Hog1 nuclear localization during adaptation to osmotic stress under glucose-limited conditions than under glucose-rich conditions. We proposed and verified that in glucose-limited environment, glycolysis intermediates (representing “reserve flux”) were limited, which required cells to express more glycerol-production enzymes for stress adaptation. Consequently, the regulatory mechanism of osmoresponse was derived in the presence and absence of such reserve flux. Further experiments suggested that this reserve flux-dependent stress-defense strategy may be a general principle under nutrient-limited environments.
