Ubiquitin Homeostasis Is Disrupted in TDP-43 and FUS Cell Models of ALS
Natalie E. Farrawell,
Luke McAlary,
Jeremy S. Lum,
Christen G. Chisholm,
Sadaf T. Warraich,
Ian P. Blair,
Kara L. Vine,
Darren N. Saunders,
Justin J. Yerbury
Affiliations
Natalie E. Farrawell
Illawarra Health and Medical Research Institute, Wollongong, NSW 2522, Australia; Molecular Horizons and School of Chemistry and Molecular Bioscience, Science Medicine and Health Faculty, University of Wollongong, Northfields Avenue, Wollongong, NSW 2522, Australia
Luke McAlary
Illawarra Health and Medical Research Institute, Wollongong, NSW 2522, Australia; Molecular Horizons and School of Chemistry and Molecular Bioscience, Science Medicine and Health Faculty, University of Wollongong, Northfields Avenue, Wollongong, NSW 2522, Australia
Jeremy S. Lum
Illawarra Health and Medical Research Institute, Wollongong, NSW 2522, Australia; Molecular Horizons and School of Chemistry and Molecular Bioscience, Science Medicine and Health Faculty, University of Wollongong, Northfields Avenue, Wollongong, NSW 2522, Australia
Christen G. Chisholm
Illawarra Health and Medical Research Institute, Wollongong, NSW 2522, Australia; Molecular Horizons and School of Chemistry and Molecular Bioscience, Science Medicine and Health Faculty, University of Wollongong, Northfields Avenue, Wollongong, NSW 2522, Australia
Sadaf T. Warraich
Centre for Motor Neuron Disease Research, Department of Biomedical Sciences, Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney, NSW 2109, Australia
Ian P. Blair
Centre for Motor Neuron Disease Research, Department of Biomedical Sciences, Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney, NSW 2109, Australia
Kara L. Vine
Illawarra Health and Medical Research Institute, Wollongong, NSW 2522, Australia; Molecular Horizons and School of Chemistry and Molecular Bioscience, Science Medicine and Health Faculty, University of Wollongong, Northfields Avenue, Wollongong, NSW 2522, Australia
Darren N. Saunders
School of Medical Sciences, Faculty of Medicine, UNSW, Sydney, NSW 2052, Australia
Justin J. Yerbury
Illawarra Health and Medical Research Institute, Wollongong, NSW 2522, Australia; Molecular Horizons and School of Chemistry and Molecular Bioscience, Science Medicine and Health Faculty, University of Wollongong, Northfields Avenue, Wollongong, NSW 2522, Australia; Corresponding author
Summary: A major feature of amyotrophic lateral sclerosis (ALS) pathology is the accumulation of ubiquitin (Ub) into intracellular inclusions. This sequestration of Ub may reduce the availability of free Ub, disrupting Ub homeostasis and ultimately compromising cellular function and survival. We previously reported significant disturbance of Ub homeostasis in neuronal-like cells expressing mutant SOD1. Here, we show that Ub homeostasis is also perturbed in neuronal-like cells expressing either TDP-43 or FUS. The expression of mutant TDP-43 and mutant FUS led to UPS dysfunction, which was associated with a redistribution of Ub and depletion of the free Ub pool. Redistribution of Ub is also a feature of sporadic ALS, with an increase in Ub signal associated with inclusions and no compensatory increase in Ub expression. Together, these findings suggest that alterations to Ub homeostasis caused by the misfolding and aggregation of ALS-associated proteins play an important role in the pathogenesis of ALS.
