Honeybee Royal Jelly and Nobiletin Stimulate CRE-Mediated Transcription in ERK-Independent and -Dependent Fashions, Respectively, in PC12D Cells

Hironori Fujiwara; Ako Kogure; Masahiro Sakamoto; Tohru Yamakuni; Yoshihiro Mimaki; Kiyoshi Murata; Nobuyuki Hitomi; Kikuji Yamaguchi; Yasushi Ohizumi

Journal of Pharmacological Sciences (Jan 2011)

Honeybee Royal Jelly and Nobiletin Stimulate CRE-Mediated Transcription in ERK-Independent and -Dependent Fashions, Respectively, in PC12D Cells

Hironori Fujiwara,
Ako Kogure,
Masahiro Sakamoto,
Tohru Yamakuni,
Yoshihiro Mimaki,
Kiyoshi Murata,
Nobuyuki Hitomi,
Kikuji Yamaguchi,
Yasushi Ohizumi

Affiliations

Hironori Fujiwara: Department of Anti-dementia Functional Food Development, Graduate School of Engineering, Tohoku University, Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan
Ako Kogure: Department of Anti-dementia Functional Food Development, Graduate School of Engineering, Tohoku University, Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan
Masahiro Sakamoto: Department of Anti-dementia Functional Food Development, Graduate School of Engineering, Tohoku University, Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan
Tohru Yamakuni: Laboratory of Pharmacotherapy, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba-ku, Sendai 980-8578, Japan
Yoshihiro Mimaki: Laboratory of Medicinal Plant Science, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192-0392, Japan
Kiyoshi Murata: Japan Royal Jelly (JRJ) Co., Ltd., R&D Center, Ganbara, Kami-machi, Kami-gun, Miyagi 981-4263, Japan
Nobuyuki Hitomi: Japan Royal Jelly (JRJ) Co., Ltd., R&D Center, Ganbara, Kami-machi, Kami-gun, Miyagi 981-4263, Japan
Kikuji Yamaguchi: Department of Anti-dementia Functional Food Development, Graduate School of Engineering, Tohoku University, Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan; Japan Royal Jelly (JRJ) Co., Ltd., Kyobasi, Chuo-ku, Tokyo 104-0031, Japan; Apis Cerana Research Institute, Yunnan Agriculture University, Yunnan 650201, China
Yasushi Ohizumi: Department of Anti-dementia Functional Food Development, Graduate School of Engineering, Tohoku University, Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan; Yokohama College of Pharmacy, 601 Matano-cho, Tozuka-ku, Yokohama 245-0066, Japan; Department of Pharmacokinetics and Pharmacodynamics, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan; Corresponding author (affiliation #1). [email protected]

Journal volume & issue: Vol. 116, no. 4
pp. 384 – 387

Abstract

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Abstract.: To prove the pharmacological actions of honeybee royal jelly (RJ) on the nervous system, we examined the effects of RJ on CRE-mediated transcription. RJ increased CRE-mediated transcription in PC12D cells. Moreover, CRE-mediated transcriptional activity by RJ was enhanced by nobiletin. U0126, a MEK inhibitor, inhibited CRE-mediated transcription by combining RJ plus nobiletin without affecting transcription by RJ alone. These results suggest that RJ stimulates CRE-mediated transcription via an ERK-independent cascade, whereas the increasing CRE-mediated transcriptional effect by nobiletin is dependent on ERK phosphorylation. Combining RJ plus nobiletin may activate effectively neuronal functions via enhancement of CRE-mediated transcription. Keywords:: honeybee royal jelly, CRE-mediated transcription, Alzheimer’s disease

Published in Journal of Pharmacological Sciences

ISSN: 1347-8613 (Print)
Publisher: Elsevier
Country of publisher: Japan
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: http://www.journals.elsevier.com/journal-of-pharmacological-sciences

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