Nanometre-Scale Visualization of Chemical Parameter Changes by T<sub>1</sub>-Weighted ODMR Imaging Using a Fluorescent Nanodiamond

Takahiro Fujisaku; Ryuji Igarashi; Masahiro Shirakawa

doi:10.3390/chemosensors8030068

Chemosensors (Aug 2020)

Nanometre-Scale Visualization of Chemical Parameter Changes by T<sub>1</sub>-Weighted ODMR Imaging Using a Fluorescent Nanodiamond

Takahiro Fujisaku,
Ryuji Igarashi,
Masahiro Shirakawa

Affiliations

Takahiro Fujisaku: Department of Molecular Engineering, Graduate School of Engineering, Kyoto University, Nishikyo-Ku, Kyoto 615-8510, Japan
Ryuji Igarashi: Institute for Quantum Life Science, National Institutes for Quantum and Radiological Science and Technology, Anagawa 4-9-1, Inage-ku, Chiba 263-8555, Japan
Masahiro Shirakawa: Department of Molecular Engineering, Graduate School of Engineering, Kyoto University, Nishikyo-Ku, Kyoto 615-8510, Japan

DOI: https://doi.org/10.3390/chemosensors8030068
Journal volume & issue: Vol. 8, no. 3
p. 68

Abstract

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The dynamics of physical parameters in cells is strongly related to life phenomena; thus, a method to monitor and visualize them on a single-organelle scale would be useful to reveal unknown biological processes. We demonstrate real-time nanometre-scale T1-weighted imaging using a fluorescent nanodiamond. We explored optically detected magnetic resonance (ODMR) contrast at various values of interval laser pulse (τ), showing that sufficient contrast is obtained by appropriate selection of τ. By this method, we visualized nanometre-scale pH changes using a functionalized nanodiamond whose T1 has a dependence on pH conditions.

Published in Chemosensors

ISSN: 2227-9040 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry: Biochemistry
Website: http://www.mdpi.com/journal/chemosensors

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