A novel method for evaluating activity of transient receptor potential channels using a cellular dielectric spectroscopy

Kanako Miyano; Katsuya Ohbuchi; Yuka Sudo; Kouichiro Minami; Toru Yokoyama; Masahiro Yamamoto; Miaki Uzu; Miki Nonaka; Seiji Shiraishi; Hiroaki Murata; Yoshikazu Higami; Yasuhito Uezono

Journal of Pharmacological Sciences (Aug 2020)

A novel method for evaluating activity of transient receptor potential channels using a cellular dielectric spectroscopy

Kanako Miyano,
Katsuya Ohbuchi,
Yuka Sudo,
Kouichiro Minami,
Toru Yokoyama,
Masahiro Yamamoto,
Miaki Uzu,
Miki Nonaka,
Seiji Shiraishi,
Hiroaki Murata,
Yoshikazu Higami,
Yasuhito Uezono

Affiliations

Kanako Miyano: Division of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan
Katsuya Ohbuchi: Tsumura Research Labs, Tumura & Co., 3586 Yoshiwara, Ami-machi, Inashiki-gun, Ibaraki 300-1192, Japan
Yuka Sudo: Division of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan; Laboratory of Molecular Pathology and Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba 278-8510, Japan
Kouichiro Minami: Division of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan; Emergency Life-Saving Technique Academy of Tokyo (ELSTA TOKYO), Hachioji-shi, Tokyo 192-0364, Japan; Department of Anesthesiology and Critical Care Medicine, Jichi Medical University, Tochigi 329-0498, Japan
Toru Yokoyama: Division of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan; Emergency Life-Saving Technique Academy of Tokyo (ELSTA TOKYO), Hachioji-shi, Tokyo 192-0364, Japan; Department of Anesthesiology and Critical Care Medicine, Jichi Medical University, Tochigi 329-0498, Japan
Masahiro Yamamoto: Tsumura Research Labs, Tumura & Co., 3586 Yoshiwara, Ami-machi, Inashiki-gun, Ibaraki 300-1192, Japan
Miaki Uzu: Division of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan
Miki Nonaka: Division of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan
Seiji Shiraishi: Division of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan
Hiroaki Murata: Department of Anesthesiology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852-8501, Japan
Yoshikazu Higami: Laboratory of Molecular Pathology and Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba 278-8510, Japan; Translational Research Center, Research Institute of Science and Technology, Tokyo University of Science, Chiba 278-8510, Japan
Yasuhito Uezono: Division of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan; Translational Research Center, Research Institute of Science and Technology, Tokyo University of Science, Chiba 278-8510, Japan; Division of Supportive Care Research, Exploratory Oncology Research & Clinical Trial Center, National Cancer Center, Tokyo 104-0045, Japan; Innovation Center for Supportive, Palliative and Psychosocial Care, National Cancer Center Hospital, Tokyo 104-0045, Japan; Department of Comprehensive Oncology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852-8523, Japan; Corresponding author. Division of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan. Fax: +81 3 3542 0688.

Journal volume & issue: Vol. 143, no. 4
pp. 320 – 324

Abstract

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Cellular dielectric spectroscopy (CDS) is a novel technology enabling pharmacological evaluation of multiple receptor types with a label-free cell-based assay. We evaluated activities of a family of ligand-gated channels, transient receptor potential vanilloid 1 (TRPV1) and transient receptor potential ankyrin 1 (TRPA1) channels by an electrical impedance-based biosensor (CellKey™ system) using CDS. Measures of both potency (EC50) and efficacy (Emax) of these agonists with CellKey™ were almost identical to those made using the traditional Ca2+ influx assay in TRPV1- or TRPA1-expressing cells, suggesting that CellKey™ is a simpler and easier means of evaluating TRP activities.

Published in Journal of Pharmacological Sciences

ISSN: 1347-8613 (Print)
Publisher: Elsevier
Country of publisher: Japan
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: http://www.journals.elsevier.com/journal-of-pharmacological-sciences

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