The first bioactive (angiotensin-converting enzyme-inhibitory) peptide isolated from pearl matrix protein

Chaoyi Wu; Zehui Yin; Yayu Wang; Xinjiani Chen; Bailei Li; Qin Wang; Liping Yao; Zhen Zhang; Xiaojun Liu; Rongqing Zhang

Heliyon (Apr 2024)

The first bioactive (angiotensin-converting enzyme-inhibitory) peptide isolated from pearl matrix protein

Chaoyi Wu,
Zehui Yin,
Yayu Wang,
Xinjiani Chen,
Bailei Li,
Qin Wang,
Liping Yao,
Zhen Zhang,
Xiaojun Liu,
Rongqing Zhang

Affiliations

Chaoyi Wu: Key Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Ocean University, Ministry ofAgriculture, Shanghai, 201306, China
Zehui Yin: Key Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Ocean University, Ministry ofAgriculture, Shanghai, 201306, China
Yayu Wang: Department of Biotechnology and Biomedicine, Yangtze Delta Region Institute of Tsinghua University, Zhejiang, 314000, China
Xinjiani Chen: Department of Biotechnology and Biomedicine, Yangtze Delta Region Institute of Tsinghua University, Zhejiang, 314000, China
Bailei Li: Department of Biotechnology and Biomedicine, Yangtze Delta Region Institute of Tsinghua University, Zhejiang, 314000, China
Qin Wang: Department of Biotechnology and Biomedicine, Yangtze Delta Region Institute of Tsinghua University, Zhejiang, 314000, China
Liping Yao: Department of Biotechnology and Biomedicine, Yangtze Delta Region Institute of Tsinghua University, Zhejiang, 314000, China
Zhen Zhang: Department of Biotechnology and Biomedicine, Yangtze Delta Region Institute of Tsinghua University, Zhejiang, 314000, China; Zhejiang Provincial Key Laboratory of Applied Enzymology, Yangtze Delta Region Institute of Tsinghua University, 705 Yatai Road, Jiaxing, 314006, China
Xiaojun Liu: Department of Biotechnology and Biomedicine, Yangtze Delta Region Institute of Tsinghua University, Zhejiang, 314000, China; Taizhou Innovation Center, Yangtze Delta Region Institute of Tsinghua University, Zhejiang, 318000, China; Zhejiang Provincial Key Laboratory of Applied Enzymology, Yangtze Delta Region Institute of Tsinghua University, 705 Yatai Road, Jiaxing, 314006, China; Corresponding author. Department of Biotechnology and Biomedicine, Yangtze Delta Region Institute of Tsinghua University, Zhejiang, 314000, China.
Rongqing Zhang: Department of Biotechnology and Biomedicine, Yangtze Delta Region Institute of Tsinghua University, Zhejiang, 314000, China; Taizhou Innovation Center, Yangtze Delta Region Institute of Tsinghua University, Zhejiang, 318000, China; Zhejiang Provincial Key Laboratory of Applied Enzymology, Yangtze Delta Region Institute of Tsinghua University, 705 Yatai Road, Jiaxing, 314006, China; Corresponding author. Department of Biotechnology and Biomedicine, Yangtze Delta Region Institute of Tsinghua University, Zhejiang, 314000, China.

Journal volume & issue: Vol. 10, no. 7
p. e28060

Abstract

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In this research, we unveil the medical potential of pearls by identifying a novel bioactive peptide within them for the first time. The peptide, termed KKCHFWPFPW, emerges as a pioneering angiotensin I-converting enzyme (ACE) inhibitor, originating from the pearl matrix of Pinctada fucata. Employing quadrupole time-of-flight mass spectrometry, this peptide was meticulously selected and pinpointed. With a molecular weight of 1417.5 Da and a theoretical isoelectric point of 9.31, its inhibitory potency was demonstrated through a half-maximal inhibitory concentration (IC50) of 4.17 μM, established via high-performance liquid chromatography. The inhibition of ACE by this peptide was found to be competitive, as revealed by Lineweaver–Burk plot analysis, where an increase in peptide concentration correlated with an enhanced rate of ACE inhibition. To delve into the interaction between KKCHFWPFPW and ACE, molecular docking simulations were conducted using the Maestro 2022-1 Glide software, shedding light on the inhibitory mechanism. This investigation suggests that peptides derived from the P. martensii pearl matrix hold promise as a novel source for antihypertensive agents.

Published in Heliyon

ISSN: 2405-8440 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Science: Science (General); Social Sciences: Social sciences (General)
Website: https://www.cell.com/heliyon/home

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