This article reports fractal dimension analysis applied to soil CO2 fluxes measured in an Italian seismic area. The work was carried out with the use of a calibrated flux chamber unit. The fractal dimension (FD) from isotropic variograms was used as a method to understand related scale-dependent phenomena. The aim was to investigate the spatial variability of CO2 flux measurements in four directions (horizontal, vertical, 45° and 135° directions) related to different distances between the measuring points and from a fault. High fractal dimension values were found (2.5 ≤ FD ≤ 3.0). These imply strong anti-persistent behavior near to and far from the fault. Lower fractal dimensions were addressed at longer distances from the fault.