Bacteriocin-Nanoconjugates (Bac10307-AgNPs) Biosynthesized from <i>Lactobacillus acidophilus</i>-Derived Bacteriocins Exhibit Enhanced and Promising Biological Activities

Arif Jamal Siddiqui; Mitesh Patel; Mohd Adnan; Sadaf Jahan; Juhi Saxena; Mohammed Merae Alshahrani; Abdelmushin Abdelgadir; Fevzi Bardakci; Manojkumar Sachidanandan; Riadh Badraoui; Mejdi Snoussi; Allal Ouhtit

doi:10.3390/pharmaceutics15020403

Pharmaceutics (Jan 2023)

Bacteriocin-Nanoconjugates (Bac10307-AgNPs) Biosynthesized from <i>Lactobacillus acidophilus</i>-Derived Bacteriocins Exhibit Enhanced and Promising Biological Activities

Arif Jamal Siddiqui,
Mitesh Patel,
Mohd Adnan,
Sadaf Jahan,
Juhi Saxena,
Mohammed Merae Alshahrani,
Abdelmushin Abdelgadir,
Fevzi Bardakci,
Manojkumar Sachidanandan,
Riadh Badraoui,
Mejdi Snoussi,
Allal Ouhtit

Affiliations

Arif Jamal Siddiqui: Department of Biology, College of Science, University of Ha’il, Ha’il P.O. Box 2440, Saudi Arabia
Mitesh Patel: Department of Biotechnology, Parul Institute of Applied Sciences and Centre of Research for Development, Parul University, Vadodara 391760, India
Mohd Adnan: Department of Biology, College of Science, University of Ha’il, Ha’il P.O. Box 2440, Saudi Arabia
Sadaf Jahan: Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, Al Majmaah 11952, Saudi Arabia
Juhi Saxena: Department of Biotechnology, University Institute of Biotechnology, Chandigarh University, Gharuan, NH-95, Ludhiana-Chandigarh State Hwy, Punjab 140413, India
Mohammed Merae Alshahrani: Department of Clinical Laboratory Sciences, Faculty of Applied Medial Sciences, Najran University, 1988, Najran 61441, Saudi Arabia
Abdelmushin Abdelgadir: Department of Biology, College of Science, University of Ha’il, Ha’il P.O. Box 2440, Saudi Arabia
Fevzi Bardakci: Department of Biology, College of Science, University of Ha’il, Ha’il P.O. Box 2440, Saudi Arabia
Manojkumar Sachidanandan: Department of Oral Radiology, College of Dentistry, University of Ha’il, Ha’il P.O. Box 2440, Saudi Arabia
Riadh Badraoui: Department of Biology, College of Science, University of Ha’il, Ha’il P.O. Box 2440, Saudi Arabia
Mejdi Snoussi: Department of Biology, College of Science, University of Ha’il, Ha’il P.O. Box 2440, Saudi Arabia
Allal Ouhtit: Department of Biological and Environmental Sciences, College of Arts and Sciences, Qatar University, Doha P.O. Box 2713, Qatar

DOI: https://doi.org/10.3390/pharmaceutics15020403
Journal volume & issue: Vol. 15, no. 2
p. 403

Abstract

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The proteinaceous compounds produced by lactic acid bacteria are called bacteriocins and have a wide variety of bioactive properties. However, bacteriocin’s commercial availability is limited due to short stability periods and low yields. Therefore, the objective of this study was to synthesize bacteriocin-derived silver nanoparticles (Bac10307-AgNPs) extracted from Lactobacillus acidophilus (L. acidophilus), which may have the potential to increase the bioactivity of bacteriocins and overcome the hurdles. It was found that extracted and purified Bac10307 had a broad range of stability for both temperature (20–100 °C) and pH (3–12). Further, based on Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS–PAGE) analysis, its molecular weight was estimated to be 4.2 kDa. The synthesized Bac10307-AgNPs showed a peak of surface plasmon resonance at 430 nm λmax. Fourier transform infrared (FTIR) confirmed the presence of biological moieties, and transmission electron microscopy (TEM) coupled with Energy dispersive X-Ray (EDX) confirmed that AgNPs were spherical and irregularly shaped, with a size range of 9–20 nm. As a result, the Bac10307-AgNPs displayed very strong antibacterial activity with MIC values as low as 8 μg/mL for Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa), when compared to Bac10307 alone. In addition, Bac10307-AgNPs demonstrated promising in vitro antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50 = 116.04 μg/mL) and in vitro cytotoxicity against human liver cancer cells (HepG2) (IC50 = 135.63 μg/mL), more than Bac10307 alone (IC50 = 139.82 μg/mL against DPPH and 158.20 μg/mL against HepG2). Furthermore, a protein–protein molecular docking simulation study of bacteriocins with target proteins of different biological functions was also carried out in order to ascertain the interactions between bacteriocins and target proteins.

Published in Pharmaceutics

ISSN: 1999-4923 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine: Pharmacy and materia medica
Website: http://www.mdpi.com/journal/pharmaceutics/

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