Preparation of Teased Nerve Fibers from Rat Sciatic Nerve
Jinkun Wen,
Lixia Li,
Dandan Tan,
Jiasong Guo
Affiliations
Jinkun Wen
Department of Histology and Embryology, Southern Medical University, Guangdong, Guangzhou, China
Lixia Li
Department of Histology and Embryology, Southern Medical University, Guangdong, Guangzhou, China
Dandan Tan
Department of Histology and Embryology, Southern Medical University, Guangdong, Guangzhou, China
Jiasong Guo
Department of Histology and Embryology, Southern Medical University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Southern Medical University, Guangdong, Guangzhou, China, Institute of Bone Biology, Academy of Orthopedics, Guangdong, Guangzhou, China
Compared to tissue sectioning techniques, the technique of teasing single nerve fibers provides a better way to understand the structures of myelin sheaths and axons of the peripheral myelinated nerves. This protocol describes a method for preparation of teased single nerve fibers from rat sciatic nerve. In this protocol, fixed nerves are teased into single individual fibers and arranged onto adhesion microscope slides for further immuno-staining.
