Metal-Ion Assisted Imprinted Hydrogels For Recognition Of Lysozyme

Abstract

Hydrogels exhibiting selectivity towards lysozyme were produced by metal-ion assisted-imprinting technology. A metal-chelate monomer N-vinyl-2-pyrrolidone is pre-assembled with the template molecule via assistance of Cu(II) ions and co-polymerized with 2-hydroxyethyl methacrylate. Lysozyme imprinted hydrogels were characterized by Fourier transform infrared spectroscopy, swelling tests, scanning electron microscopy. The conditions for the optimum adsorption capacity of the hydrogels towards lysozyme were found out by investigating the effects of initial concentration of lysozyme, medium pH, contact time and ionic strength. Maximum adsorption of lysozyme on poly(hydroxyethyl methacrylate-co-N-vinyl pyrrolidone) hydrogel was found to be 12.25 mg/g for 1.0 mg/mL initial concentration of lysozyme at 25.0°C with an optimal pH of 7.0. After ten adsorption-desorption cycles with the same hydrogel, the lysozyme adsorption capacity decreased by 13.80%.

Keywords

• hydrogels
• lysozyme
• metal ion coordination
• molecular imprinting
• hidrojeller
• lizozim
• metal iyon koordinasyonu
• moleküler baskılama