Study of content alterations of peroxidase, ascorbate peroxidase and catalase activity in two soybean cultivars (Glycine max L. merr) under water stress

Abstract

Different cultivars of one species activate various enzymes such as superoxide dismutase, catalase, peroxidase, glutathione reductase and ascorbate peroxidase in order to defense against the water stress.These enzymes have important role in plant defense against the reactive oxygene species. In this study, behavior different of peroxidase, ascorbate peroxidase and catalase and effects of water stress on activity of them against drought and flooding were investigated in two soybean cultivars (Glycine max L. cv. Pershing and cv. DPX). An experiment was carried out under potting conditions and 4 treatments (20, 40, 60 and 80% of water saturation capacity) were used. Antioxidant enzymes indicated differences with stress and organ. Peroxidase activity increased in nodule and root under drought and flooding stress but in leaf, peroxidase activity increased in lower water contents (20 and 40%) and in DPX, significant difference did not shown in the all. In both studied organs, catalase activity increased in flooding and drought, in DPX and Pershing, repectively but significant difference did not shown in other treatment. In the leaf, ascorbate peroxidase activity decreased with decreasing in irrigation but in root, its activity indicated significant increaseing in flooding and drought, in DPX and Pershing, repectively. Results indicated that enzymetic activity can vary by cultivar, stress and organ type, too.

Keywords

• ascorbate peroxidase
• catalase
• peroxidase
• soybean and water stress