Ameliorative effect of Leucas cephalotes extract on isoniazid and rifampicin induced hepatotoxicity

Abstract

Objective: To investigate ameliorative effect of alcoholic extract of whole herb of Lucas cephalotes (L. cephalotes) on isoniazid and rifampicin induced hepatotoxicity. Methods: Hepatoprotective potential of alcoholic extract of L. cephalotes was ascertained at 200 mg/kg and 400 mg/kg in Sprague Dawley rats. Animals were intoxicated with isoniazid and rifampicin (100 mg/kg each, i.p.) for 21 d. Assessment was done on the basis of liver function test (serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, alkaline phosphatase and bilirubin); effect on enzymes involved in oxidative stress (lipid peroxidase, superoxide dismutase, glutathione and catalase) and histology of liver were assessed as well. Comparison among different treatment group was done using One way ANOVA followed by Benferroni's t test. Results: It was observed that at both of the selected doses extract exhibited significant ameliorative effect. In animals treated with the extract at both doses, serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, alkaline phosphatase, bilirubin and lipid peroxidase level was found to be significantly less (P<0.01) and superoxide dismutase, glutathione and catalase level was significantly high (P<0.01) as compared to intoxicated animals. Conclusions: From the present study it can be concluded that alcoholic extract of L. cephalotes possesses significant hepatoprotective potential against isoniazid and rifampicin induced heptoxicity.

Keywords

• Leucas cephalotes
• Isoniazid
• Refampicin
• Hepatoprotective
• Oxidative stress