Nature Communications (May 2018)

Intravital imaging by simultaneous label-free autofluorescence-multiharmonic microscopy

  • Sixian You,
  • Haohua Tu,
  • Eric J. Chaney,
  • Yi Sun,
  • Youbo Zhao,
  • Andrew J. Bower,
  • Yuan-Zhi Liu,
  • Marina Marjanovic,
  • Saurabh Sinha,
  • Yang Pu,
  • Stephen A. Boppart

DOI
https://doi.org/10.1038/s41467-018-04470-8
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 9

Abstract

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Label-free and real-time visualization of the tumor microenvironment is attractive but challenging. Here the authors present an approach for simultaneous autofluorescence functional imaging and second/third harmonic generation imaging of structural features, using a single excitation source.