Lab protocol for investigating impacts of climate change on frogs
Muhammad Saeed,
Muhammad Rais,
Syeda Maria Ali,
Durraj Nadeem Khosa,
Ayesha Akram,
Waseem Ahmed,
Sumbul Gill
Affiliations
Muhammad Saeed
Herpetology Lab, Department of Wildlife Management, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Punjab, Pakistan, 46000; Corresponding author.
Muhammad Rais
Herpetology Lab, Department of Wildlife Management, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Punjab, Pakistan, 46000
Syeda Maria Ali
Assistant Professor, Environmental Sciences, International Islamic University Islamabad, Islamabad, 44000
Durraj Nadeem Khosa
Fatima Jinnah Women University Rawalpindi, Rawalpindi, Pakistan, 46000
Ayesha Akram
Herpetology Lab, Department of Wildlife Management, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Punjab, Pakistan, 46000
Waseem Ahmed
Herpetology Lab, Department of Wildlife Management, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Punjab, Pakistan, 46000
Sumbul Gill
Herpetology Lab, Department of Wildlife Management, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Punjab, Pakistan, 46000
We developed a method to investigate impacts of temperature (elevated) on breeding, growth and development in endemic frogs under laboratory conditions. The method provides details on housing and rearing of larvae, taking various important measurements and observing developmental deformities. The method could also be applied to rescue approach or head-start program for amphibian species experiencing climate change elsewhere in the world.• Rearing of larvae to investigate effects of temperature on larvae• Measurement of data on growth and development• Rescue/ head-start program
