Investigation of early neoplastic transformation and premalignant biology using genetically engineered organoid models

Hua Zhao; Casey Collet; Dongzi Peng; Uttam K. Sinha; De-Chen Lin

Computational and Structural Biotechnology Journal (Jan 2022)

Investigation of early neoplastic transformation and premalignant biology using genetically engineered organoid models

Hua Zhao,
Casey Collet,
Dongzi Peng,
Uttam K. Sinha,
De-Chen Lin

Affiliations

Hua Zhao: Center for Craniofacial Molecular Biology, Herman Ostrow School of Dentistry, and Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA 90033, USA
Casey Collet: Center for Craniofacial Molecular Biology, Herman Ostrow School of Dentistry, and Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA 90033, USA
Dongzi Peng: Center for Craniofacial Molecular Biology, Herman Ostrow School of Dentistry, and Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA 90033, USA; Department of Gastroenterology, The Second Xiangya Hospital of Central South University, Changsha 410011, China
Uttam K. Sinha: Department of Otolaryngology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA; Corresponding authors at: Department of Otolaryngology, Keck School of Medicine, University of Southern California, 1537 Norfolk Street, Suite 5800, Los Angeles, CA 90033, USA (U.K. Sinha). Center for Craniofacial Molecular Biology, University of Southern California, 2250 Alcazar Street – CSA 207D, Los Angeles, CA 90033, USA (D.-C. Lin).
De-Chen Lin: Center for Craniofacial Molecular Biology, Herman Ostrow School of Dentistry, and Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA 90033, USA; Corresponding authors at: Department of Otolaryngology, Keck School of Medicine, University of Southern California, 1537 Norfolk Street, Suite 5800, Los Angeles, CA 90033, USA (U.K. Sinha). Center for Craniofacial Molecular Biology, University of Southern California, 2250 Alcazar Street – CSA 207D, Los Angeles, CA 90033, USA (D.-C. Lin).

Journal volume & issue: Vol. 20
pp. 5309 – 5315

Abstract

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Organoid modeling is a powerful, robust and efficient technology faithfully preserving physiological and pathological characteristics of tissues of origin. Recently, substantial advances have been made in applying genetically engineered organoid models to study early tumorigenesis and premalignant biology. These efforts promise to identify novel avenues for early cancer detection, intervention and prevention. Here, we highlight significant advancements in the functional characterization of early genomic and epigenomic events during neoplastic evolution using organoid modeling, discuss the application of the lineage-tracing methodology in organoids to study cancer cells-of-origin, and review future opportunities for further development and improvement of organoid modeling of cancer precursors.

Published in Computational and Structural Biotechnology Journal

ISSN: 2001-0370 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Technology: Chemical technology: Biotechnology
Website: https://www.journals.elsevier.com/computational-and-structural-biotechnology-journal

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