Science Journal of University of Zakho (Jun 2013)
In Vitro Rooting Of Hybrid Rose (Rosa Carolina L.)Shoots and Subsequent Establishment
Abstract
A successful in vitro propagation system has been developed for the valuable ornamental plant hybrid rose (Rosa carolina L.) by in vitro culture of shoot tips from mature plants. First shoot tip explants were excised and cultured on basal medium of Murashige and Skoog (1962) containing several concentrations of BAP and Kinetin. Multiple shoot formation of up to 3 shoots was obtained on MS medium supplemented with 4.0 mg/l BAP. Induced microshoots was then tested for root initiation on full MS medium supplemented with different concentrations of Indolbutyric acid (IBA) and Naphthalene acetic acid (NAA) and the best root formation was observed on a medium containing 1.0 and 2.0 mg/l of IBA and 2.0 mg/l of NAA. The regenerated plants were successfully transplanted to the greenhouse and appeared similar characteristics to the parent plants in morphologic features.
