Structural Dynamics (Jul 2017)
Structural enzymology using X-ray free electron lasers
- Christopher Kupitz,
- Jose L. Olmos Jr.,
- Mark Holl,
- Lee Tremblay,
- Kanupriya Pande,
- Suraj Pandey,
- Dominik Oberthür,
- Mark Hunter,
- Mengning Liang,
- Andrew Aquila,
- Jason Tenboer,
- George Calvey,
- Andrea Katz,
- Yujie Chen,
- Max O. Wiedorn,
- Juraj Knoska,
- Alke Meents,
- Valerio Majriani,
- Tyler Norwood,
- Ishwor Poudyal,
- Thomas Grant,
- Mitchell D. Miller,
- Weijun Xu,
- Aleksandra Tolstikova,
- Andrew Morgan,
- Markus Metz,
- Jose M. Martin-Garcia,
- James D. Zook,
- Shatabdi Roy-Chowdhury,
- Jesse Coe,
- Nirupa Nagaratnam,
- Domingo Meza,
- Raimund Fromme,
- Shibom Basu,
- Matthias Frank,
- Thomas White,
- Anton Barty,
- Sasa Bajt,
- Oleksandr Yefanov,
- Henry N. Chapman,
- Nadia Zatsepin,
- Garrett Nelson,
- Uwe Weierstall,
- John Spence,
- Peter Schwander,
- Lois Pollack,
- Petra Fromme,
- Abbas Ourmazd,
- George N. Phillips Jr.,
- Marius Schmidt
Affiliations
- Christopher Kupitz
- Physics Department, University of Wisconsin-Milwaukee, 3135 N. Maryland Ave, Milwaukee, Wisconsin 53211, USA
- Jose L. Olmos Jr.
- Department of BioSciences, Rice University, 6100 Main Street, Houston, Texas 77005, USA
- Mark Holl
- Department of Physics, Arizona State University, Tempe, Arizona 85287, USA
- Lee Tremblay
- Marbles Inc., 1900 Belvedere Pl, Westfield, Indiana 46074, USA
- Kanupriya Pande
- Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
- Suraj Pandey
- Physics Department, University of Wisconsin-Milwaukee, 3135 N. Maryland Ave, Milwaukee, Wisconsin 53211, USA
- Dominik Oberthür
- Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
- Mark Hunter
- Linac Coherent Light Source, Stanford Linear Accelerator Center (SLAC) National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA
- Mengning Liang
- Linac Coherent Light Source, Stanford Linear Accelerator Center (SLAC) National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA
- Andrew Aquila
- Linac Coherent Light Source, Stanford Linear Accelerator Center (SLAC) National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA
- Jason Tenboer
- Physics Department, University of Wisconsin-Milwaukee, 3135 N. Maryland Ave, Milwaukee, Wisconsin 53211, USA
- George Calvey
- Department of Applied and Engineering Physics, Cornell University, 254 Clark Hall, Ithaca, New York 14853, USA
- Andrea Katz
- Department of Applied and Engineering Physics, Cornell University, 254 Clark Hall, Ithaca, New York 14853, USA
- Yujie Chen
- Department of Applied and Engineering Physics, Cornell University, 254 Clark Hall, Ithaca, New York 14853, USA
- Max O. Wiedorn
- Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
- Juraj Knoska
- Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
- Alke Meents
- University of Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany
- Valerio Majriani
- Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
- Tyler Norwood
- Physics Department, University of Wisconsin-Milwaukee, 3135 N. Maryland Ave, Milwaukee, Wisconsin 53211, USA
- Ishwor Poudyal
- Physics Department, University of Wisconsin-Milwaukee, 3135 N. Maryland Ave, Milwaukee, Wisconsin 53211, USA
- Thomas Grant
- Hauptman-Woodward Institute, State University of New York at Buffalo, 700 Ellicott Street, Buffalo, New York 14203, USA
- Mitchell D. Miller
- Department of BioSciences, Rice University, 6100 Main Street, Houston, Texas 77005, USA
- Weijun Xu
- Department of BioSciences, Rice University, 6100 Main Street, Houston, Texas 77005, USA
- Aleksandra Tolstikova
- Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
- Andrew Morgan
- Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
- Markus Metz
- Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
- Jose M. Martin-Garcia
- School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University, Tempe, Arizona 85287-1604, USA
- James D. Zook
- School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University, Tempe, Arizona 85287-1604, USA
- Shatabdi Roy-Chowdhury
- School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University, Tempe, Arizona 85287-1604, USA
- Jesse Coe
- School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University, Tempe, Arizona 85287-1604, USA
- Nirupa Nagaratnam
- School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University, Tempe, Arizona 85287-1604, USA
- Domingo Meza
- School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University, Tempe, Arizona 85287-1604, USA
- Raimund Fromme
- School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University, Tempe, Arizona 85287-1604, USA
- Shibom Basu
- School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University, Tempe, Arizona 85287-1604, USA
- Matthias Frank
- Lawrence Livermore National Laboratory, Livermore, California 94550, USA
- Thomas White
- Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
- Anton Barty
- Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
- Sasa Bajt
- Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
- Oleksandr Yefanov
- Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
- Henry N. Chapman
- Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
- Nadia Zatsepin
- Department of Physics, Arizona State University, Tempe, Arizona 85287, USA
- Garrett Nelson
- Department of Physics, Arizona State University, Tempe, Arizona 85287, USA
- Uwe Weierstall
- Department of Physics, Arizona State University, Tempe, Arizona 85287, USA
- John Spence
- Department of Physics, Arizona State University, Tempe, Arizona 85287, USA
- Peter Schwander
- Physics Department, University of Wisconsin-Milwaukee, 3135 N. Maryland Ave, Milwaukee, Wisconsin 53211, USA
- Lois Pollack
- Department of Applied and Engineering Physics, Cornell University, 254 Clark Hall, Ithaca, New York 14853, USA
- Petra Fromme
- School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University, Tempe, Arizona 85287-1604, USA
- Abbas Ourmazd
- Physics Department, University of Wisconsin-Milwaukee, 3135 N. Maryland Ave, Milwaukee, Wisconsin 53211, USA
- George N. Phillips Jr.
- Department of BioSciences, Rice University, 6100 Main Street, Houston, Texas 77005, USA
- Marius Schmidt
- Physics Department, University of Wisconsin-Milwaukee, 3135 N. Maryland Ave, Milwaukee, Wisconsin 53211, USA
- DOI
- https://doi.org/10.1063/1.4972069
- Journal volume & issue
-
Vol. 4,
no. 4
pp. 044003 – 044003-7
Abstract
Mix-and-inject serial crystallography (MISC) is a technique designed to image enzyme catalyzed reactions in which small protein crystals are mixed with a substrate just prior to being probed by an X-ray pulse. This approach offers several advantages over flow cell studies. It provides (i) room temperature structures at near atomic resolution, (ii) time resolution ranging from microseconds to seconds, and (iii) convenient reaction initiation. It outruns radiation damage by using femtosecond X-ray pulses allowing damage and chemistry to be separated. Here, we demonstrate that MISC is feasible at an X-ray free electron laser by studying the reaction of M. tuberculosis ß-lactamase microcrystals with ceftriaxone antibiotic solution. Electron density maps of the apo-ß-lactamase and of the ceftriaxone bound form were obtained at 2.8 Å and 2.4 Å resolution, respectively. These results pave the way to study cyclic and non-cyclic reactions and represent a new field of time-resolved structural dynamics for numerous substrate-triggered biological reactions.
