The complete chloroplast genome of a Cymbidium Tortisepalum (Orchidaceae) male mutant

Abstract

The chloroplast (cp) genome of natural male mutant Cymbidium tortisepalum ‘Guanshihe’ has been characterized using Illumina pair-end sequencing technology. The complete cp genome was 149,830 bp in length, containing a large single-copy region (LSC) of 85,131 bp and a small single-copy region (SSC) of 13,275 bp, which were separated by a pair of 25,712 bp inverted repeat regions (IRs). The genome contained 130 genes, with 111 unique genes, including 77 protein-coding genes, 30 tRNA genes, and 4 rRNA genes. The overall GC content is 37.09% with the values of the LSC, SSC, and IR regions are 34.40%, 29.63%, and 43.45%, respectively. Further, phylogenetic analysis suggested that the plastome of C. tortisepalum male mutant ‘Guanshihe’ is close to sequenced C. sinense, C. kanran, C. tortisepalum, and C. ensifolium plastomes.

Keywords

• cymbidium tortisepalum
• female sterility
• plastome
• phylogeny
• orchidaceae