Generation of a TRPV1 knockout human pluripotent stem cell line (WAe009-A-U) using CRISPR/Cas9

Yuhong Cao; Ling Tang; Jun Su; Hao Wang; Ping Liang; Kuang Rong; Tingyu Gong

Stem Cell Research (Oct 2023)

Generation of a TRPV1 knockout human pluripotent stem cell line (WAe009-A-U) using CRISPR/Cas9

Yuhong Cao,
Ling Tang,
Jun Su,
Hao Wang,
Ping Liang,
Kuang Rong,
Tingyu Gong

Affiliations

Yuhong Cao: Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Institute of Translational Medicine, Zhejiang University, Hangzhou 310029, China; NMPA Key Laboratory for Animal Alternative Testing Technology of Cosmetis, Zhejiang Institute for Food and Drug Control, Hangzhou 310052, China
Ling Tang: Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Institute of Translational Medicine, Zhejiang University, Hangzhou 310029, China
Jun Su: Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Institute of Translational Medicine, Zhejiang University, Hangzhou 310029, China
Hao Wang: Prenatal Diagnosis Center, Hangzhou Women's Hospital, Hangzhou 310008, China
Ping Liang: Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Institute of Translational Medicine, Zhejiang University, Hangzhou 310029, China; Corresponding authors at: Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China (P. Liang and T. Gong).
Kuang Rong: NMPA Key Laboratory for Animal Alternative Testing Technology of Cosmetis, Zhejiang Institute for Food and Drug Control, Hangzhou 310052, China; Corresponding authors at: Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China (P. Liang and T. Gong).
Tingyu Gong: Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Shulan International Medical College, Zhejiang Shuren University, Hangzhou 310015, China; Corresponding authors at: Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China (P. Liang and T. Gong).

Journal volume & issue: Vol. 72
p. 103202

Abstract

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The transient receptor potential vanilloid subfamily 1 (TRPV1) is a polymodal nociceptor that is highly expressed in sensory nerves. Activation of TRPV1 receptors excites primary afferent nociceptors by opening cation channels, allowing the influx of Na+ and Ca2+ ions into the cytoplasm. Here, a TRPV1 knockout human embryonic stem cell line was generated using the CRISPR/Cas9 genome-editing technology to further study the function of TRPV1. The cell line confirmed with normal pluripotency and karyotype.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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