The influence of overexpressions of microRNA-375 on the expression of thymic stromal lymphopoietin and IL-4, IL-13 in allergic rhinitis mice

Abstract

Objective: To detect the expression of thymic stromal lymphopoietin (TSLP) and IL-4, IL- 13 (interleukin-4, interleukin-13) through overexpression of microRNA-375 in allergic rhinitis (AR) mice. Methods: AR mice models with overexpression of miRNA375 were induced by ovalbumin (OVA) in Lentivirus overexpression vector AR group (Overexpression vector+OVA), lentivirus control vector AR group (Control vector+OVA) as well as AR (OVA) group. While saline was administered in control (Saline) group, nasal mucosa of each group were collected for further use. Relative expressions of TSLP and IL-4, IL-13 mRNA and proteins were detected by quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and western blot analysis. Results: Real-time fluorescence quantitative method verified the overexpression of miRNA375 in allergic rhinitis group, and the same method has been adopted to verify the expressions of IL-4 and IL-13 genes in the overexpression group. The relative expressions of TSLP and IL-4, IL-13 mRNA and protein decreased in overexpression of microRNA-375 nasal mucosa compared with AR group (P<0.05). Conclusions: The results indicated that the lentivirus in the nasal mucosa of mice with allergic rhinitis may affect the expression of IL-4, IL-13 and TSLP, and the expression of lentivirus in the nasal mucosa of mice with allergic rhinitis is different from that in the miR-375 lentivirus group. The expression of lentivirus in the nasal mucosa of mice with allergic rhinitis was different.

Keywords

• Allergic rhinitis; MicroRNA375; Thymic stromal lymphopoietin; IL-4; IL-13