Assay of Ampicillin in capsule Dosage Form Manufactured by Different Pharmaceutical Manufacturing Factories available in Iraq

Abstract

The assay of Ampicillin is carried out by HPLC (High performance liquid chromatography) system to calculate the weight of the active constituent of the drug in this dosage form. In this work we study six samples of Ampicillin 250 mg capsule manufactured by different pharm- aceutical companies available in Iraq to evaluate the content of drug in this dosage form. This has been achieved by making calibration curve of different concentrations of stock solution, using standard ampicillin tri hydrate (Reference Standard Ampicillin) USP, we obtained different reading of the area under the peak (AUP) which followed straight – line equation.

Keywords

• Assay, (HPLC) High performance liquid chromatography, (AUP) Area under the peak, Ampicillin Trihydrate (AMP. Tri. H).