Dataset on CRISPR/Cas9 system targeting hydrogenase genes in Rhodobacter johrii MAY2 isolate
Leo Agustin F. Barcelo,
Nacita B. Lantican,
Aprill P. Manalang,
Jey-R S. Ventura
Affiliations
Leo Agustin F. Barcelo
Biomaterials and Environmental Engineering Laboratory, Department of Engineering Science, College of Engineering and Agro-Industrial Technology, University of the Philippines Los Baños, College, Laguna 4031 Philippines
Nacita B. Lantican
Microbiology Division, Institute of Biological Sciences, College of Arts and Sciences, University of the Philippines Los Baños, College, Laguna 4031 Philippines
Aprill P. Manalang
Genetics and Molecular Biology Division, Institute of Biological Sciences, College of Arts and Sciences, University of the Philippines Los Baños, College, Laguna 4031 Philippines
Jey-R S. Ventura
Biomaterials and Environmental Engineering Laboratory, Department of Engineering Science, College of Engineering and Agro-Industrial Technology, University of the Philippines Los Baños, College, Laguna 4031 Philippines; Corresponding author.
This dataset contains the gene sequences of the small and large sub-unit of the hydrogenase enzyme obtained from the annotated genome of Rhodobacter johrii MAY2. The whole genome sequence of the isolate was performed using SEED genome viewer on the Rapid Annotation using the Subsystem Technology (RAST) platform. Concurrently, guide RNA sequences and primers were meticulously crafted using the CHOPCHOP v.3.0 web tool, specifically designed for the precise editing and amplification of the target genes. The primers were optimized via gradient PCR to determine appropriate amplification conditions. Furthermore, the guide RNA was tested via in-vitro cleavage assay, gauging its efficacy in cleaving the intended target genes. The dataset, including the optimization and the cleavage assay, was deposited in Mendeley Data with DOI no: 10.17632/rcx3mcssnx.2.
