A paper-based colorimetric molecular test for SARS-CoV-2 in saliva

Josiah Levi Davidson; Jiangshan Wang; Murali Kannan Maruthamuthu; Andres Dextre; Ana Pascual-Garrigos; Suraj Mohan; Sai Venkata Sravan Putikam; Fujr Osman Ibrahim Osman; Darby McChesney; Jordan Seville; Mohit S. Verma

Biosensors and Bioelectronics: X (Dec 2021)

A paper-based colorimetric molecular test for SARS-CoV-2 in saliva

Josiah Levi Davidson,
Jiangshan Wang,
Murali Kannan Maruthamuthu,
Andres Dextre,
Ana Pascual-Garrigos,
Suraj Mohan,
Sai Venkata Sravan Putikam,
Fujr Osman Ibrahim Osman,
Darby McChesney,
Jordan Seville,
Mohit S. Verma

Affiliations

Josiah Levi Davidson: Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, 47907, USA; Birck Nanotechnology Center, Purdue University, West Lafayette, IN, 47907, USA
Jiangshan Wang: Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, 47907, USA; Birck Nanotechnology Center, Purdue University, West Lafayette, IN, 47907, USA
Murali Kannan Maruthamuthu: Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, 47907, USA; Birck Nanotechnology Center, Purdue University, West Lafayette, IN, 47907, USA
Andres Dextre: Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, 47907, USA; Birck Nanotechnology Center, Purdue University, West Lafayette, IN, 47907, USA
Ana Pascual-Garrigos: Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, 47907, USA; Birck Nanotechnology Center, Purdue University, West Lafayette, IN, 47907, USA
Suraj Mohan: Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, 47907, USA; Birck Nanotechnology Center, Purdue University, West Lafayette, IN, 47907, USA
Sai Venkata Sravan Putikam: Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, 47907, USA; Birck Nanotechnology Center, Purdue University, West Lafayette, IN, 47907, USA
Fujr Osman Ibrahim Osman: Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, 47907, USA; Birck Nanotechnology Center, Purdue University, West Lafayette, IN, 47907, USA
Darby McChesney: PortaScience, Inc, Moorestown, NJ, 08057, USA
Jordan Seville: PortaScience, Inc, Moorestown, NJ, 08057, USA
Mohit S. Verma: Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, 47907, USA; Birck Nanotechnology Center, Purdue University, West Lafayette, IN, 47907, USA; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, 47907, USA; Corresponding author. 225 S. University St, West Lafayette, IN, 47907, USA.

Journal volume & issue: Vol. 9
p. 100076

Abstract

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Herein, we describe the development of a paper-based device to detect nucleic acids of pathogens of interest in complex samples using loop-mediated isothermal amplification (LAMP) by producing a colorimetric response visible to the human eye. To demonstrate the utility of this device in emerging public health emergencies, we developed and optimized our device to detect SARS-CoV-2 in human saliva without preprocessing. The resulting device was capable of detecting the virus within 60 min and had an analytical sensitivity of 97% and a specificity of 100% with a limit of detection of 200 genomic copies/μL of patient saliva using image analysis. The device consists of a configurable number of reaction zones constructed of Grade 222 chromatography paper separated by 20 mil polystyrene spacers attached to a Melinex® backing via an ARclean® double-sided adhesive. The resulting device is easily configurable to detect multiple targets and has the potential to detect a variety of pathogens simply by changing the LAMP primer sets.

Published in Biosensors and Bioelectronics: X

ISSN: 2590-1370 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Technology: Chemical technology: Biotechnology
Website: https://www.journals.elsevier.com/biosensors-and-bioelectronics-x

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