Canonical PRC1 controls sequence-independent propagation of Polycomb-mediated gene silencing

Hagar F. Moussa; Daniel Bsteh; Ramesh Yelagandula; Carina Pribitzer; Karin Stecher; Katarina Bartalska; Luca Michetti; Jingkui Wang; Jorge A. Zepeda-Martinez; Ulrich Elling; Jacob I. Stuckey; Lindsey I. James; Stephen V. Frye; Oliver Bell

doi:10.1038/s41467-019-09628-6

Nature Communications (Apr 2019)

Canonical PRC1 controls sequence-independent propagation of Polycomb-mediated gene silencing

Hagar F. Moussa,
Daniel Bsteh,
Ramesh Yelagandula,
Carina Pribitzer,
Karin Stecher,
Katarina Bartalska,
Luca Michetti,
Jingkui Wang,
Jorge A. Zepeda-Martinez,
Ulrich Elling,
Jacob I. Stuckey,
Lindsey I. James,
Stephen V. Frye,
Oliver Bell

Affiliations

Hagar F. Moussa: Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter (VBC)
Daniel Bsteh: Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter (VBC)
Ramesh Yelagandula: Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter (VBC)
Carina Pribitzer: Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter (VBC)
Karin Stecher: Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter (VBC)
Katarina Bartalska: Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter (VBC)
Luca Michetti: Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter (VBC)
Jingkui Wang: Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC)
Jorge A. Zepeda-Martinez: Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter (VBC)
Ulrich Elling: Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter (VBC)
Jacob I. Stuckey: Center for Integrative Chemical Biology and Drug Discovery, Division of Chemical Biology and Medicinal Chemistry, UNC Eshelman School of Pharmacy, University of North Carolina at Chapel Hill
Lindsey I. James: Center for Integrative Chemical Biology and Drug Discovery, Division of Chemical Biology and Medicinal Chemistry, UNC Eshelman School of Pharmacy, University of North Carolina at Chapel Hill
Stephen V. Frye: Center for Integrative Chemical Biology and Drug Discovery, Division of Chemical Biology and Medicinal Chemistry, UNC Eshelman School of Pharmacy, University of North Carolina at Chapel Hill
Oliver Bell: Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter (VBC)

DOI: https://doi.org/10.1038/s41467-019-09628-6
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 12

Abstract

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Polycomb Repressive Complexes PRC1 and PRC2 catalyse distinct chromatin modifications to promote gene silencing. Here the authors use reversible tethering of Polycomb proteins to ectopic sites in mouse ESCs and find that canonical but not variant PRC1 can trigger sequence-independent propagation of Polycomb-mediated transcriptional repression.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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