OPTIMIZATION OF THE MOLECULAR DIAGNOSIS OF ALLERGIC DISEASES THROUGH THE USE OF CCD-BLOCKERS

Abstract

Background. The development of methodological approaches aimed to overcome non-specific reactions caused by the presence of antibodies to CCD, in order to assign a rational molecular diagnosis is relevant and economically reasonable. Goal. Optimization of the tactics of serological examination of patients with multiple reactions to allergens. Material and Methods. 2197 patients were examined for IgE antibodies to plant and household respiratory allergens, of these 84 patients with multiple reactions were retested on blots similar to those used during the initial examination after the absorption of serum by CCD-blocker. After that 33 patients underwent component-resolved diagnosis for birch and timothy grass allergens. Results. The prognostic value of the absence of IgE antibodies blocking by CCD blocker reached 91.9%, meaning that a constant reaction class after blocking the serum with a probability of 91.9% did not require molecular diagnostics. The predictive value of the complete disappearance of the marker after blocking was 86.2%, so antibodies to recombinant allergens could be detected only in 13.8% patients. Conclusion. The use of CCD-blocker in patients with polysensitization allows eliminating false positive reactions and optimizing the subsequent prescription of adequate molecular diagnostics.

Keywords

• allergy
• cross-reacting carbohydrate determinants
• CCD
• multiple reactions
• component diagnostics