DIFFERENT CHARACTERISTICS OF INFECTION WITH MYCOBACTERIA IN GRANULOMA CELLS FROM MICE WITH LATENT TUBERCULOSIS INFECTION AND IN BONE MARROW AND PERITONEAL MACROPHAGES AFTER BCG VACCINE APPLICATION IN VITRO

Abstract

The aim of the study was to compare the content of BCG-mycobacteria in granulomas obtained from various organs of BALB/c mice with latent tuberculosis after in vivo exposure to BCG vaccine and in mouse bone marrow and peritoneal macrophages after BCG infection in vitro. The granuloma cells obtained from mice through 20 days, one and two months after their BCG-infecting in vivo were differed with respect to both the number of granulomas with macrophages containing the defined numbers of BCG-mycobacteria and the quantity of cells with the defined numbers of bacilli in granulomas. However, in the preparations obtained from each mice, granuloma macrophages contained solitary BCGmycobacteria. At the same time, a shorter acute infection of mouse peritoneal and bone marrow macrophages by BCG vaccine resulted in considerable growth of bacilli in the host cells for 5 days in the culture in vitro. Therefore, granuloma macrophages could control BCG infection both in mice with latent tuberculosis in vivo and in the ex vivo culture, on the contrary, the bone marrow and peritoneal macrophages were not capable to control the BCG infection in the culture in vitro.

Keywords

• latent tuberculosis infection
• bacillus calmette–gu rin vaccine
• infectiousness of cells with bcg-mycobacteria
• macrophages