Nature Communications (Jan 2016)
Reprogramming triggers endogenous L1 and Alu retrotransposition in human induced pluripotent stem cells
- Sabine Klawitter,
- Nina V. Fuchs,
- Kyle R. Upton,
- Martin Muñoz-Lopez,
- Ruchi Shukla,
- Jichang Wang,
- Marta Garcia-Cañadas,
- Cesar Lopez-Ruiz,
- Daniel J. Gerhardt,
- Attila Sebe,
- Ivana Grabundzija,
- Sylvia Merkert,
- Patricia Gerdes,
- J. Andres Pulgarin,
- Anja Bock,
- Ulrike Held,
- Anett Witthuhn,
- Alexandra Haase,
- Balázs Sarkadi,
- Johannes Löwer,
- Ernst J. Wolvetang,
- Ulrich Martin,
- Zoltán Ivics,
- Zsuzsanna Izsvák,
- Jose L. Garcia-Perez,
- Geoffrey J. Faulkner,
- Gerald G. Schumann
Affiliations
- Sabine Klawitter
- Division of Medical Biotechnology, Paul-Ehrlich-Institute
- Nina V. Fuchs
- Division of Medical Biotechnology, Paul-Ehrlich-Institute
- Kyle R. Upton
- Mater Research Institute, University of Queensland
- Martin Muñoz-Lopez
- Department of Human DNA Variability, Pfizer/University of Granada and Andalusian Regional Government Center for Genomics and Oncology (GENYO), PTS Granada
- Ruchi Shukla
- Mater Research Institute, University of Queensland
- Jichang Wang
- Max-Delbrück-Center for Molecular Medicine
- Marta Garcia-Cañadas
- Department of Human DNA Variability, Pfizer/University of Granada and Andalusian Regional Government Center for Genomics and Oncology (GENYO), PTS Granada
- Cesar Lopez-Ruiz
- Department of Human DNA Variability, Pfizer/University of Granada and Andalusian Regional Government Center for Genomics and Oncology (GENYO), PTS Granada
- Daniel J. Gerhardt
- Mater Research Institute, University of Queensland
- Attila Sebe
- Division of Medical Biotechnology, Paul-Ehrlich-Institute
- Ivana Grabundzija
- Max-Delbrück-Center for Molecular Medicine
- Sylvia Merkert
- Department of Cardiac, Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Thoracic, Transplantation, and Vascular Surgery; REBIRTH, Cluster of Excellence, Hannover Medical School
- Patricia Gerdes
- Mater Research Institute, University of Queensland
- J. Andres Pulgarin
- Department of Human DNA Variability, Pfizer/University of Granada and Andalusian Regional Government Center for Genomics and Oncology (GENYO), PTS Granada
- Anja Bock
- Division of Medical Biotechnology, Paul-Ehrlich-Institute
- Ulrike Held
- Division of Medical Biotechnology, Paul-Ehrlich-Institute
- Anett Witthuhn
- Department of Cardiac, Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Thoracic, Transplantation, and Vascular Surgery; REBIRTH, Cluster of Excellence, Hannover Medical School
- Alexandra Haase
- Department of Cardiac, Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Thoracic, Transplantation, and Vascular Surgery; REBIRTH, Cluster of Excellence, Hannover Medical School
- Balázs Sarkadi
- Department of Biophysics and Radiation Biology, Semmelweis University
- Johannes Löwer
- Division of Medical Biotechnology, Paul-Ehrlich-Institute
- Ernst J. Wolvetang
- Australian Institute for Bioengineering and Nanotechnology, The University of Queensland
- Ulrich Martin
- Department of Cardiac, Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Thoracic, Transplantation, and Vascular Surgery; REBIRTH, Cluster of Excellence, Hannover Medical School
- Zoltán Ivics
- Division of Medical Biotechnology, Paul-Ehrlich-Institute
- Zsuzsanna Izsvák
- Max-Delbrück-Center for Molecular Medicine
- Jose L. Garcia-Perez
- Department of Human DNA Variability, Pfizer/University of Granada and Andalusian Regional Government Center for Genomics and Oncology (GENYO), PTS Granada
- Geoffrey J. Faulkner
- Mater Research Institute, University of Queensland
- Gerald G. Schumann
- Division of Medical Biotechnology, Paul-Ehrlich-Institute
- DOI
- https://doi.org/10.1038/ncomms10286
- Journal volume & issue
-
Vol. 7,
no. 1
pp. 1 – 14
Abstract
Genetic and epigenetic abnormalities have been found to result from reprogramming of differentiated cells into human induced pluripotent stem cells (hiPSCs). Here, Klawitter et al.identify endogenous L1, Alu and SVA mobilization during reprogramming, highlighting the risk of insertional mutagens in hiPSCs.
