Nature Communications (Oct 2021)

A chemical probe based on the PreQ1 metabolite enables transcriptome-wide mapping of binding sites

  • Sumirtha Balaratnam,
  • Curran Rhodes,
  • Desta Doro Bume,
  • Colleen Connelly,
  • Christopher C. Lai,
  • James A. Kelley,
  • Kamyar Yazdani,
  • Philip J. Homan,
  • Danny Incarnato,
  • Tomoyuki Numata,
  • John S. Schneekloth Jr

DOI
https://doi.org/10.1038/s41467-021-25973-x
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 15

Abstract

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The small modified nucleotide PreQ1 binds to the PreQ1 riboswitch and regulates gene expression by inducing RNA conformation change. Here the authors design and characterize a specific preQ1-derived probe by x-ray crystallography, mass spectral analysis and transcriptome-wide using Chem-CLIP.