Assessment of genetic diversity in maize (Zea mays L.) inbred lines on the basis of soluble seed proteins using SDS-PAGE

Abstract

In the present investigation 25 genotypes of maize collected from different sources were characterized for the soluble seed proteins using sodium dodecyl sulphate polyacrylamide gelelectrophoresis (SDS-PAGE). The total protein bands were separated on 12% polyacrylamide gel using standard protocols. Highest number of bands observed was 14 in inbred HUZQPM-1.Highest coefficient of similarity matrix was0.69whereas lowest was 0.04. The dendrogram prepared by using UPGMA clustering method separated the whole genotypes into two main groups; I and II. On the basis of present investigation, it is observed that there is low variability present among quality protein maize inbreds.

Keywords

• Maize
• Genetic diversity
• soluble seed protein
• SDS-PAGE