Comprehensive morphometric assessment of deltoid muscle development in children: A cross-sectional studyResearch in context
Teresinha Evangelista,
Malick Kandji,
Emmanuelle Lacene,
Anaïs Chanut,
Mai Thao Bui,
Rudy Marty,
Laurent Buffat,
Kenneth Knoblauch,
Brian B. Rudkin,
Norma Beatriz Romero
Affiliations
Teresinha Evangelista
AP-HP, H. Pitié-Salpêtrière, Institut de Myologie, Unité de Morphologie Neuromusculaire, France; AP-HP, H. Pitié-Salpêtrière, Centre de référence des maladies neuromusculaires Nord/Est/Ile de France, France; Sorbonne Université, INSERM, Institut de Myologie, Centre de Recherche en Myologie, France; Corresponding author. AP-HP, H. Pitié-Salpêtrière, Institut de Myologie, Unité de Morphologie Neuromusculaire, France.
Malick Kandji
CARPACCIO.cloud, Lyon, France
Emmanuelle Lacene
AP-HP, H. Pitié-Salpêtrière, Institut de Myologie, Unité de Morphologie Neuromusculaire, France
Anaïs Chanut
AP-HP, H. Pitié-Salpêtrière, Institut de Myologie, Unité de Morphologie Neuromusculaire, France
Mai Thao Bui
AP-HP, H. Pitié-Salpêtrière, Institut de Myologie, Unité de Morphologie Neuromusculaire, France
Rudy Marty
CARPACCIO.cloud, Lyon, France
Laurent Buffat
AltraBio, Lyon, France
Kenneth Knoblauch
Univ Lyon, Université Claude Bernard Lyon 1, Inserm, Stem-cell & Brain Research Institute, Bron, France; National Centre for Optics, Vision and Eye Care, Faculty of Health and Social Sciences, University of South-Eastern Norway, Kongsberg, Norway
Brian B. Rudkin
CARPACCIO.cloud, Lyon, France; Univ Lyon, Université Claude Bernard Lyon 1, Inserm, Stem-cell & Brain Research Institute, Bron, France; Shanghai Jiao Tong University Institute of Medical Genetics, Shanghai Children's Hospital, Shanghai, PR China; Corresponding author. Univ Lyon, Université Claude Bernard Lyon 1, Inserm, Stem-cell & Brain Research Institute, Bron, France.
Norma Beatriz Romero
AP-HP, H. Pitié-Salpêtrière, Institut de Myologie, Unité de Morphologie Neuromusculaire, France; AP-HP, H. Pitié-Salpêtrière, Centre de référence des maladies neuromusculaires Nord/Est/Ile de France, France; Sorbonne Université, INSERM, Institut de Myologie, Centre de Recherche en Myologie, France
Summary: Background: Normative values for different morphometric parameters of muscle fibres during paediatric development, i.e. from 0 to 18 years, are currently unavailable. They would be of major importance to accurately evaluate pathological changes and could be used as reference biomarkers for evaluating treatment response in clinical trials, or physiological adjustments in sports or ageing. Methods: Data were derived from 482 images with a total of 33 094 fibres from 10 μm cross-sections of snap-frozen muscle from 83 deltoid muscle biopsies from patients, 0–18 years, without neuromuscular pathology stained with ATPase 9.4. Data was acquired and analysed with patented image analysis algorithms from “CARPACCIO.cloud”. Several parameters were extracted or calculated, including cross-sectional area (CSA), fibre type, circularity, as well as the Minimum diameter of Feret (MinFeret). Findings: This study illustrates changes in quantitative parameters for muscle morphology over the course of paediatric development and the pivotal changes occurring around puberty. Only fibre size parameters (MinFeret, CSA) are dependent on gender, and only after puberty. All other parameters vary in a similar manner for females and males. The proportion of type 1 fibres is essentially constant from birth to age 10, decreasing to ≈40% by age 18. Circularity decreases with age, to plateau after age 10 for both fibre types. Interpretation: Normative values and reference charts for muscle fibre types in this age range have been generated to allow comparison of data from patients in pathology laboratories working on neuromuscular diseases. Funding: BPI FRANCE, PULSALYS, Association de l’Institut de Myologie, French National Research Agency (ANR), LABEX CORTEX of Université de Lyon.
