Aquatic Sciences and Engineering (Jan 2023)
The Effect of Improper Packaging on Moisture and Fatty Acid Composition in Frozen Bluefin Tuna (Thynnus Thynnus)
Abstract
This article investigates the effect of improper packaging and freezer burn on the moisture content and fatty acid profile of frozen bluefin tuna. Improper packaging caused serious freezer burn on the surface of a bluefin tuna slice during frozen storage. The moisture content of the surface affected from freezer burn and an inner part of the tuna slice was analysed. Visual examination showed that the surface of the tuna slices were dried and different from normal flesh colour. Moisture content of the frozen tuna slices dropped significantly on the surface compared to the inner part unaffected by freezer burn. Direct methylation method was successfully achieved on the sample without any lipid extraction. Separation of fatty acid methyl esters of the bluefin tuna was successfully achieved by using GC-FID 100 m column in 65 minutes. Significant changes were observed in saturated and polyunsaturated fattyacids, whereas monounsaturated fatty acids remained the same. The level of polyunsaturated fatty acids reduced by half on the surface of the flesh compared to the inner part. Among the PUFA, n3 and n6 fatty acids were greatly reduced, but more intense in n3 fatty acids.
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