Diabetes mellitus is a metabolic disorder of multiple etiology, characterized by chronic hyperglycaemia with disturbance of carbohydrate, fat, and protein metabolism resulting from defect in insulin secretion, insulin action or both. Improper lifestyle contributes to the increasing number of people affected with Type 2 diabetes mellitus (T2DM). Systematic reviews on the management of T2DM in adults through Yoga reported significant improvements in multiple modifiable indices of diabetes mellitus management including glycemic control, lipid levels, and body composition. Awareness levels of a condition among the population play a critical role in behaviour change. However, studies related to assessing the awareness and practice of Yoga for managing diseases are limited. Hence, this study was formulated with the objective of assessing the awareness level and extent of knowledge about diabetes mellitus and its management through yoga. A cross-sectional survey with a sample size of 317 was conducted using a structured questionnaire at 5 districts in Tamil Nadu and Kerala. 95% of the study population were aware of diabetes mellitus while 61.2% responded that diabetes can be prevented by regular exercise and healthy diet. 62.4% people perceived that yoga practices can prevent diabetes mellitus and 59% mentioned that regular yoga practice can help in controlling diabetes and prevent further complications. Only 13% reported to practice Yoga regularly at least three days a week. Identifying a qualified Yoga trainer was reported to be a limiting factor for regular yoga practice. The study suggested that there is a need to increase the access to qualified Yoga professionals at community level. Further large scale studies with random sampling method to assess the awareness level and practice of Yoga in different settings is indicated.