Application of green-synthesized cerium oxide nanoparticles to treat spinal cord injury and cytotoxicity evaluation on paediatric leukaemia cells

Abstract

Injury prognosis, and treatment are one of the major objectives of nursing care during the treatment of spinal cord injury (SCI). Here, we developed a novel, biocompatible Cerium oxide nanoparticles (CeO _2 -NPs) through biosynthetic approach that can be used for the treatment of spinal cord injury in nursing care. CeO _2 -NPs were green fabricated utilizing leaf extract of Azadirachta indica . The fabricated nanoparticles still maintained the cubic-structure that was revealed by the x-ray diffraction (X-RD) analysis. Transmission electron microscopy (TEM) images displayed the sphere shape of nanoparticles having 15 nm particle size. The stretching bands of Ce–O bond were noticed at 457 cm ^−1 and 451 cm ^−1 from the Raman and Fourier-transform Infrared (FT-IR) spectra, correspondingly. On the other side, biological investigations demonstrated that the single dose application of CeO _2 -NPs at nanomolar concentration is regenerative, bio-compatible and offers a considerable neuro-protective effect on the neurons in spinal-cord of an adult rat. Neuronal function retention is showed in electro-physiological (electrography) recordings and plausibility of its uses in preventing ischemic-insult is revealed from the assay of oxidative injury. This work explored the possibility of usage of prepared CeO _2 -NPs for SCI treatment. Furthermore, examination of green fabricated CeO _2 -NPs on paediatric leukaemia (CCRF-SB) cells were found to potentiate cytotoxicity indicating their therapeutic potential for future treatment of paediatric cancer. On the other hand, the current study also highlights the potential effect of CeO _2 -NPs on treatment of SCI through nano-based therapy.

Keywords

• CeO2-NPs
• spinal cord injury
• green synthesis
• pediatric leukemia