Frozen Autoclaved Sorghum Enhanced Colonic Fermentation and Lower Visceral Fat Accumulation in Rats
Samanthi W. Pelpolage,
Atsushi Yoshida,
Ryuji Nagata,
Kenichiro Shimada,
Naoki Fukuma,
Hiroki Bochimoto,
Tetsuo Hamamoto,
Michiyo Hoshizawa,
Koichi Nakano,
Kyu-Ho Han,
Michihiro Fukushima
Affiliations
Samanthi W. Pelpolage
Department of Life and Food Sciences, Obihiro University of Agriculture and Veterinary Medicine, West 2-11, Inada, Obihiro 080–8555, Hokkaido, Japan
Atsushi Yoshida
Department of Life and Food Sciences, Obihiro University of Agriculture and Veterinary Medicine, West 2-11, Inada, Obihiro 080–8555, Hokkaido, Japan
Ryuji Nagata
Department of Life and Food Sciences, Obihiro University of Agriculture and Veterinary Medicine, West 2-11, Inada, Obihiro 080–8555, Hokkaido, Japan
Kenichiro Shimada
Department of Life and Food Sciences, Obihiro University of Agriculture and Veterinary Medicine, West 2-11, Inada, Obihiro 080–8555, Hokkaido, Japan
Naoki Fukuma
Department of Life and Food Sciences, Obihiro University of Agriculture and Veterinary Medicine, West 2-11, Inada, Obihiro 080–8555, Hokkaido, Japan
Hiroki Bochimoto
Division of Aerospace Medicine, Department of Cell Physiology, The Jikei University School of Medicine, 3-25-8 Nishishimbashi, Minatoku, Tokyo 105-8461, Japan
Tetsuo Hamamoto
U.S. Grains Council, 11th Floor, Toranomon Denki Building No. 3, 1-2-20 Toranomon, Minato-ku, Tokyo 105-0001, Japan
Michiyo Hoshizawa
U.S. Grains Council, 11th Floor, Toranomon Denki Building No. 3, 1-2-20 Toranomon, Minato-ku, Tokyo 105-0001, Japan
Koichi Nakano
Nakano Industry Co., Asahishinmachi 33-25 Takamatsu, Kagawa 760-0064, Japan
Kyu-Ho Han
Department of Life and Food Sciences, Obihiro University of Agriculture and Veterinary Medicine, West 2-11, Inada, Obihiro 080–8555, Hokkaido, Japan
Michihiro Fukushima
Department of Life and Food Sciences, Obihiro University of Agriculture and Veterinary Medicine, West 2-11, Inada, Obihiro 080–8555, Hokkaido, Japan
As raw sorghum is not able to influence considerable colonic fermentation despite its higher resistant starch (RS) content, our study aimed to investigate the effects of frozen autoclaved sorghum on colonic fermentation. Fischer 344 rats were fed frozen cooked refined (S-Rf) and whole (S-Wh) sorghum diets and were compared against α-corn starch (CON) and high amylose starch (HAS) fed rats for zoometric parameters, cecal biochemical and microbiological parameters. Sorghum fed rats exhibited significantly lower feed intake and visceral adipose tissue mass compared to CON. Bacterial alpha diversity was significantly higher in the sorghum fed rats compared to HAS and the two sorghum fed groups clustered together, separately from HAS and CON in the beta diversity plot. Serum non-High Density Lipoprotein cholesterol and total cholesterol in S-Rf group were significantly lower compared to CON, while total fecal bile excretion was also significantly higher in the two sorghum fed groups. Lower visceral adiposity was correlated with lower feed intake, RS content ingested and cecal short chain fatty acid (SCFA) contents. Thus, higher RS inflow to the colon via frozen autoclaved sorghum might have influenced colonic fermentation of RS and the resultant SCFA might have influenced lower adiposity as manifested by the lower body weight gain.