Data on the phylogenetic typing, integron gene cassette array analysis, multi-drug resistance analysis and correlation between antimicrobial resistance determinants in Klebsiella strains
Hao Wu,
Mingyu Wang,
Yuqing Liu,
Xinhua Wang,
Yunkun Wang,
Jinxing Lu,
Hai Xu
Affiliations
Hao Wu
State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, Jinan 250100, Shandong, PR China
Mingyu Wang
State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, Jinan 250100, Shandong, PR China
Yuqing Liu
Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong, PR China
Xinhua Wang
School of Environmental Science and Engineering, Shandong University, Jinan 250100, Shandong, PR China
Yunkun Wang
School of Environmental Science and Engineering, Shandong University, Jinan 250100, Shandong, PR China
Jinxing Lu
State Key Laboratory for Infectious Disease Prevention and Control, and National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China
Hai Xu
State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, Jinan 250100, Shandong, PR China; Corresponding author.
The antimicrobial resistance of Klebsiella species in the poultry industry is becoming a public concern. In support our recent publication “Characterization of antimicrobial resistance in Klebsiella species isolated from chicken broilers” (Wu et al., 2016) [1], multilocus sequence typing (MLST) and gyrA PCR-RFLP assays were conducted to identify the genetic relationships between and phylogenetic groups of the 90 antimicrobial resistant Klebsiella species isolated from a commercial broiler slaughter plant in Shandong, China. In addition, PCR-RFLP was performed to identify different gene cassette arrays in class 1 and 2 integrons, and the correlations between different antimicrobial resistance determinants were analyzed. Keywords: Antimicrobial resistant Klebsiella species, Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), Gene cassette arrays of integron, Statistical analysis
