Generation of recombinant vaccinia virus and analysis of virus-induced cell death

Zhijun Liu; Kidong Kang; Francis Ka-Ming Chan

doi:10.1016/j.xpro.2021.100871

STAR Protocols (Dec 2021)

Generation of recombinant vaccinia virus and analysis of virus-induced cell death

Zhijun Liu,
Kidong Kang,
Francis Ka-Ming Chan

Affiliations

Zhijun Liu: Department of Immunology, Duke University School of Medicine, DUMC 3010, Durham, NC 27710, USA; Corresponding author
Kidong Kang: Department of Immunology, Duke University School of Medicine, DUMC 3010, Durham, NC 27710, USA
Francis Ka-Ming Chan: Department of Immunology, Duke University School of Medicine, DUMC 3010, Durham, NC 27710, USA; Corresponding author

DOI: https://doi.org/10.1016/j.xpro.2021.100871
Journal volume & issue: Vol. 2, no. 4
p. 100871

Abstract

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Summary: Vaccinia virus is a large double-stranded DNA virus that is widely used to express foreign genes from different origins. We generated recombinant vaccinia virus that expresses a viral inhibitor to examine its effect on virus-induced necroptosis. We provide a detailed protocol to describe the generation of recombinant vaccinia virus, validation of protein expression, and determination of necroptosis using live cell imaging. This approach can be adapted to examine the effect of other cell death regulators on virus-induced cell death.For complete details on the use and execution of this protocol, please refer to Liu et al. (2021).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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