Synthesis, Characterization, and Cytotoxicity Evaluation of Chlorambucil-Functionalized Mesoporous Silica Nanoparticles
Juliana Camila Fischer Karnopp,
Juliana Jorge,
Jaqueline Rodrigues da Silva,
Diego Boldo,
Kristiane Fanti Del Pino Santos,
Adriana Pereira Duarte,
Gustavo Rocha de Castro,
Ricardo Bentes de Azevedo,
Ariadna Lafourcade Prada,
Jesús Rafael Rodríguez Amado,
Marco Antonio Utrera Martines
Affiliations
Juliana Camila Fischer Karnopp
Postgraduate Program in Chemistry, Chemistry Institute, Federal University of Mato Grosso do Sul, Campo Grande 79079-900, MS, Brazil
Juliana Jorge
Postgraduate Program in Chemistry, Chemistry Institute, Federal University of Mato Grosso do Sul, Campo Grande 79079-900, MS, Brazil
Jaqueline Rodrigues da Silva
Postgraduate Program in Nanoscience and Nanotechnology, Biological Science Institute, University of Brasilia, Brasilia 70910-900, DF, Brazil
Diego Boldo
Postgraduate Program in Chemistry, Chemistry Institute, Federal University of Mato Grosso do Sul, Campo Grande 79079-900, MS, Brazil
Kristiane Fanti Del Pino Santos
Postgraduate Program in Chemistry, Chemistry Institute, Federal University of Mato Grosso do Sul, Campo Grande 79079-900, MS, Brazil
Adriana Pereira Duarte
Postgraduate Program in Chemistry, Chemistry Institute, Federal University of Mato Grosso do Sul, Campo Grande 79079-900, MS, Brazil
Gustavo Rocha de Castro
Postgraduate Program in Environmental Biotechnology, Bioscience Institute, Sao Paulo State University, Botucatu 18618-000, SP, Brazil
Ricardo Bentes de Azevedo
Postgraduate Program in Nanoscience and Nanotechnology, Biological Science Institute, University of Brasilia, Brasilia 70910-900, DF, Brazil
Ariadna Lafourcade Prada
Postgraduate Program in Biotechnology, Faculty of Pharmacy, Food and Nutrition, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil
Jesús Rafael Rodríguez Amado
Postgraduate Program in Health Sciences, Faculty of Health Sciences, Federal University of Grande Dourados, Dourados 79804-970, MS, Brazil
Marco Antonio Utrera Martines
Postgraduate Program in Chemistry, Chemistry Institute, Federal University of Mato Grosso do Sul, Campo Grande 79079-900, MS, Brazil
This study describes the synthesis and characterization of chlorambucil (CLB)-functionalized mesoporous silica nanoparticles (MSNs) for potential application in cancer therapy. The nanoparticles were designed with a diameter between 20 and 50 nm to optimize cellular uptake and avoid rapid clearance from the bloodstream. The synthesis method involved modifying a previously reported technique to reduce particle size. Successful functionalization with CLB was confirmed through various techniques, including Fourier transform infrared spectroscopy (FTIR) and elemental analysis. The cytotoxicity of the CLB-functionalized nanoparticles (MSN@NH2-CLB) was evaluated against human lung adenocarcinoma cells (A549) and colon carcinoma cells (CT26WT). The results suggest significantly higher cytotoxicity of MSN@NH2-CLB compared to unbound CLB, with improved selectivity towards cancer cells over normal cells. This suggests that MSN@NH2-CLB holds promise as a drug delivery system for targeted cancer therapy.
