Diversity and Virulence of <i>Diaporthe</i> Species Associated with Peach Trunk Diseases in China
Ying Zhou,
Wei Zhang,
Pranami D. Abeywickrama,
Zhizheng He,
Zhixiang Zhang,
Yonghua Li,
Shifang Li,
Zaifeng Fan,
Jiye Yan
Affiliations
Ying Zhou
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Wei Zhang
Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Key Laboratory of Environment Friendly Management on Fruit and Vegetable Pests in North China (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
Pranami D. Abeywickrama
Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Key Laboratory of Environment Friendly Management on Fruit and Vegetable Pests in North China (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
Zhizheng He
Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Key Laboratory of Environment Friendly Management on Fruit and Vegetable Pests in North China (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
Zhixiang Zhang
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Yonghua Li
Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Key Laboratory of Environment Friendly Management on Fruit and Vegetable Pests in North China (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
Shifang Li
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Zaifeng Fan
College of Plant Protection, China Agricultural University, Beijing 100193, China
Jiye Yan
Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Key Laboratory of Environment Friendly Management on Fruit and Vegetable Pests in North China (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
Peach (Prunus persica L.) is one of the most important and oldest stone fruits grown in China. Though Diaporthe species have more commonly been reported as plant pathogens, endophytes and saprophytes with a wide range of plant hosts, little is known about the Diaporthe species associated with peach trunk diseases in China. In the present study, forty-four Diaporthe isolates were obtained from trees with peach branch canker, shoot blight and gummosis symptoms in four provinces in China. Based on a combination of morphology and multi-locus sequence analysis of the rDNA internal transcribed spacer region (ITS), calmodulin (cal), translation elongation factor 1-α (tef1) and β-tubulin (tub2), these Diaporthe isolates were assigned to four species. Detailed descriptions and illustrations of all of the species, D. arecae, D. caulivora, D. discoidispora and D. eres, are provided. This study further reports the first host association of D. caulivora and D. discoidispora on peaches worldwide. The pathogenicity experiment results revealed that D. arecae was the most aggressive species, whereas D. discoidispora was the least aggressive on detached peach shoots. This study provides new insights into the fungi associated with peach trunk diseases in China, and the results of this study may help to facilitate routine diagnosis and planning of suitable plant disease management strategies.
