HPLC-ESI-MS<sup>n</sup> Identification and NMR Characterization of Glucosyloxybenzyl 2<i>R</i>-Benzylmalate Deriva-Tives from <i>Arundina Graminifolia</i> and Their Anti-Liver Fibrotic Effects In Vitro
Qingqing Liu,
Feiyi Sun,
Yulin Deng,
Rongji Dai,
Fang Lv
Affiliations
Qingqing Liu
Beijing Key Labrotary for Separation and Analysis in Biomedicine and Pharmaceuticals, School of Life Science, Beijing Institute of Technology, Beijing 100081, China
Feiyi Sun
Beijing Key Labrotary for Separation and Analysis in Biomedicine and Pharmaceuticals, School of Life Science, Beijing Institute of Technology, Beijing 100081, China
Yulin Deng
Beijing Key Labrotary for Separation and Analysis in Biomedicine and Pharmaceuticals, School of Life Science, Beijing Institute of Technology, Beijing 100081, China
Rongji Dai
Beijing Key Labrotary for Separation and Analysis in Biomedicine and Pharmaceuticals, School of Life Science, Beijing Institute of Technology, Beijing 100081, China
Fang Lv
Beijing Key Labrotary for Separation and Analysis in Biomedicine and Pharmaceuticals, School of Life Science, Beijing Institute of Technology, Beijing 100081, China
Four new glucosyloxybenzyl 2R-benzylmalate derivatives, named Arundinoside H (2), I (5), J (6), K (8) as well as four known compounds Arundinoside D (1), G (3), F (4), E (7) were isolated and characterized by a combination of chemical and spectroscopic methods, including HR-ESI-MS, 1D and 2D NMR experiments. Besides, 24 unreported compounds were inferred from ESI-MSn data. The anti-liver fibrotic activities of the isolates were determined as proliferation inhibition of lipopolysaccharide (LPS)-induced activation of rat hepatic stellate cells (HSC-T6). The result suggested Arundinosides D, H, F, I and K showed moderate inhibitory effects in vitro.
