HPLC-ESI-MSⁿ Identification and NMR Characterization of Glucosyloxybenzyl 2<i>R</i>-Benzylmalate Deriva-Tives from <i>Arundina Graminifolia</i> and Their Anti-Liver Fibrotic Effects In Vitro

Abstract

Four new glucosyloxybenzyl 2R-benzylmalate derivatives, named Arundinoside H (2), I (5), J (6), K (8) as well as four known compounds Arundinoside D (1), G (3), F (4), E (7) were isolated and characterized by a combination of chemical and spectroscopic methods, including HR-ESI-MS, 1D and 2D NMR experiments. Besides, 24 unreported compounds were inferred from ESI-MSn data. The anti-liver fibrotic activities of the isolates were determined as proliferation inhibition of lipopolysaccharide (LPS)-induced activation of rat hepatic stellate cells (HSC-T6). The result suggested Arundinosides D, H, F, I and K showed moderate inhibitory effects in vitro.

Keywords

• <i>Arundina graminifolia</i>
• glucosyloxybenzyl 2<i>R</i>-benzylmalates
• MSⁿ fragmentation pattern
• anti-liver fibrotic effects