Nature Communications (Nov 2017)

Mapping the sugar dependency for rational generation of a DNA-RNA hybrid-guided Cas9 endonuclease

  • Fernando Orden Rueda,
  • Michal Bista,
  • Matthew D. Newton,
  • Anne U. Goeppert,
  • M. Emanuela Cuomo,
  • Euan Gordon,
  • Felix Kröner,
  • Jon A. Read,
  • Jonathan D. Wrigley,
  • David Rueda,
  • Benjamin J. M. Taylor

DOI
https://doi.org/10.1038/s41467-017-01732-9
Journal volume & issue
Vol. 8, no. 1
pp. 1 – 11

Abstract

Read online

CRISPR-Cas9 systems are being continually improved to enhance specificity and improve functionality. Here the authors design hybrid DNA-RNA guide and tracr molecules to direct Cas9 nuclease activity with reduced off-target effects.