Study on preparation of collagen peptides from Micropterus salmoides skin and its antioxidant activity

Abstract

Objective: Collagen was extracted from the skin of Micropterus salmoides and used to explore the effect of enzyme types on the antioxidant activity of collagen peptides. Methods: Four kinds of enzymes were used, namely alkaline protease, neutral protease, papain and trypsin. The enzymatic hydrolysis of collagen Alkaline protease enzymatically hydrolyze collagen peptide （APP）, Neutral protease enzymatically hydrolyze collagen peptide （NPP）, Papain enzymatically hydrolyze collagen peptide（PP）, Trypsin hydrolysis of collagen peptide（TP）, were obtained and compared their total antioxidant activity, including reducing activity, DPPH, ·OH and O-2·free radical scavenging ability, ferrous ion chelating ability and lipid Oxidation inhibitory activity. Results: Four kinds of proteases enzymatically hydrolyze collagen peptides all have antioxidant activity, and the antioxidant capacity was positively correlated with the concentration of collagen peptides. Among them, APP has the best antioxidant activity. The total antioxidant activity and reducing activity respectively were 1.725 and 0.958, and the scavenging rates for DPPH and O-2·radical respectively were 56.35% and 38.41%, respectively. Conclusion: The collagen peptides hydrolyzed by alkaline protease have better antioxidant activity.

Keywords

• micropterus salmoides skin
• protease
• enzymatic hydrolysis
• collagen peptide
• antioxidant activity