A Novel Mix of Polyphenols and Micronutrients Reduces Adipogenesis and Promotes White Adipose Tissue Browning via UCP1 Expression and AMPK Activation
Francesca Pacifici,
Gina Malatesta,
Caterina Mammi,
Donatella Pastore,
Vincenzo Marzolla,
Camillo Ricordi,
Francesca Chiereghin,
Marco Infante,
Giulia Donadel,
Francesco Curcio,
Annalisa Noce,
Valentina Rovella,
Davide Lauro,
Manfredi Tesauro,
Nicola Di Daniele,
Enrico Garaci,
Massimiliano Caprio,
David Della-Morte
Affiliations
Francesca Pacifici
Department of Systems Medicine, University of Rome “Tor Vergata”, 00133 Rome, Italy
Gina Malatesta
Department of Systems Medicine, University of Rome “Tor Vergata”, 00133 Rome, Italy
Caterina Mammi
Laboratory of Cardiovascular Endocrinology, IRCCS San Raffaele, 00166 Rome, Italy
Donatella Pastore
Department of Human Sciences and Quality of Life Promotion, San Raffaele University, 00166 Rome, Italy
Vincenzo Marzolla
Laboratory of Cardiovascular Endocrinology, IRCCS San Raffaele, 00166 Rome, Italy
Camillo Ricordi
Cell Transplant Center, Diabetes Research Institute, University of Miami Miller School of Medicine, Miami, FL 33136, USA
Francesca Chiereghin
Department of Human Sciences and Quality of Life Promotion, San Raffaele University, 00166 Rome, Italy
Marco Infante
Department of Systems Medicine, University of Rome “Tor Vergata”, 00133 Rome, Italy
Giulia Donadel
Department of Clinical Sciences and Translational Medicine, University of Rome Tor Vergata, 00133 Rome, Italy
Francesco Curcio
Covid Internal Medicine Unit, Department of Translational Medical Sciences, AOU Federico II, University of Naples Federico II, Via S. Pansini, 5, 80131 Naples, Italy
Annalisa Noce
UOC of Internal Medicine-Center of Hypertension and Nephrology Unit, Department of Systems Medicine, University of Rome Tor Vergata, Via Montpellier 1, 00133, Rome, Italy
Valentina Rovella
Department of Systems Medicine, University of Rome “Tor Vergata”, 00133 Rome, Italy
Davide Lauro
Department of Systems Medicine, University of Rome “Tor Vergata”, 00133 Rome, Italy
Manfredi Tesauro
Department of Systems Medicine, University of Rome “Tor Vergata”, 00133 Rome, Italy
Nicola Di Daniele
Department of Systems Medicine, University of Rome “Tor Vergata”, 00133 Rome, Italy
Enrico Garaci
Department of Human Sciences and Quality of Life Promotion, San Raffaele University, 00166 Rome, Italy
Massimiliano Caprio
Laboratory of Cardiovascular Endocrinology, IRCCS San Raffaele, 00166 Rome, Italy
David Della-Morte
Department of Systems Medicine, University of Rome “Tor Vergata”, 00133 Rome, Italy
Background: Obesity is a pandemic disease characterized by excessive severe body comorbidities. Reduction in fat accumulation represents a mechanism of prevention, and the replacement of white adipose tissue (WAT) with brown adipose tissue (BAT) has been proposed as one promising strategy against obesity. In the present study, we sought to investigate the ability of a natural mixture of polyphenols and micronutrients (A5+) to counteract white adipogenesis by promoting WAT browning. Methods: For this study, we employed a murine 3T3-L1 fibroblast cell line treated with A5+, or DMSO as control, during the differentiation in mature adipocytes for 10 days. Cell cycle analysis was performed using propidium iodide staining and cytofluorimetric analysis. Intracellular lipid contents were detected by Oil Red O staining. Inflammation Array, along with qRT-PCR and Western Blot analyses, served to measure the expression of the analyzed markers, such as pro-inflammatory cytokines. Results: A5+ administration significantly reduced lipids’ accumulation in adipocytes when compared to control cells (p + inhibited cellular proliferation during the mitotic clonal expansion (MCE), the most relevant stage in adipocytes differentiation (p + significantly reduced the release of pro-inflammatory cytokines, such as IL-6 and Leptin (p p Conclusion: Overall, these results demonstrated that the synergistic effect of compounds contained in A5+ may be able to counteract adipogenesis and then obesity by inducing fat browning.
