Frontiers in Immunology (Nov 2021)
Two Duplicated Ptpn6 Homeologs Cooperatively and Negatively Regulate RLR-Mediated IFN Response in Hexaploid Gibel Carp
- Jin-Feng Tong,
- Jin-Feng Tong,
- Jin-Feng Tong,
- Li Zhou,
- Li Zhou,
- Li Zhou,
- Shun Li,
- Shun Li,
- Long-Feng Lu,
- Long-Feng Lu,
- Zhuo-Cong Li,
- Zhuo-Cong Li,
- Zhi Li,
- Zhi Li,
- Rui-Hai Gan,
- Rui-Hai Gan,
- Rui-Hai Gan,
- Cheng-Yan Mou,
- Cheng-Yan Mou,
- Cheng-Yan Mou,
- Qi-Ya Zhang,
- Qi-Ya Zhang,
- Zhong-Wei Wang,
- Zhong-Wei Wang,
- Zhong-Wei Wang,
- Xiao-Juan Zhang,
- Yang Wang,
- Yang Wang,
- Yang Wang,
- Jian-Fang Gui,
- Jian-Fang Gui,
- Jian-Fang Gui
Affiliations
- Jin-Feng Tong
- State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan, China
- Jin-Feng Tong
- College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China
- Jin-Feng Tong
- Hubei Hongshan Laboratory, Chinese Academy of Sciences, Wuhan, China
- Li Zhou
- State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan, China
- Li Zhou
- College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China
- Li Zhou
- Hubei Hongshan Laboratory, Chinese Academy of Sciences, Wuhan, China
- Shun Li
- State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan, China
- Shun Li
- College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China
- Long-Feng Lu
- State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan, China
- Long-Feng Lu
- College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China
- Zhuo-Cong Li
- State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan, China
- Zhuo-Cong Li
- College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China
- Zhi Li
- State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan, China
- Zhi Li
- Hubei Hongshan Laboratory, Chinese Academy of Sciences, Wuhan, China
- Rui-Hai Gan
- State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan, China
- Rui-Hai Gan
- College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China
- Rui-Hai Gan
- Hubei Hongshan Laboratory, Chinese Academy of Sciences, Wuhan, China
- Cheng-Yan Mou
- State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan, China
- Cheng-Yan Mou
- College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China
- Cheng-Yan Mou
- Fisheries Institute, Sichuan Academy of Agricultural Sciences, Chengdu, China
- Qi-Ya Zhang
- State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan, China
- Qi-Ya Zhang
- College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China
- Zhong-Wei Wang
- State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan, China
- Zhong-Wei Wang
- College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China
- Zhong-Wei Wang
- Hubei Hongshan Laboratory, Chinese Academy of Sciences, Wuhan, China
- Xiao-Juan Zhang
- State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan, China
- Yang Wang
- State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan, China
- Yang Wang
- College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China
- Yang Wang
- Hubei Hongshan Laboratory, Chinese Academy of Sciences, Wuhan, China
- Jian-Fang Gui
- State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan, China
- Jian-Fang Gui
- College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China
- Jian-Fang Gui
- Hubei Hongshan Laboratory, Chinese Academy of Sciences, Wuhan, China
- DOI
- https://doi.org/10.3389/fimmu.2021.780667
- Journal volume & issue
-
Vol. 12
Abstract
Src homology region 2 domain-containing phosphatase 1 (SHP1), encoded by the protein tyrosine phosphatase nonreceptor type 6 (ptpn6) gene, belongs to the family of protein tyrosine phosphatases (PTPs) and participates in multiple signaling pathways of immune cells. However, the mechanism of SHP1 in regulating fish immunity is largely unknown. In this study, we first identified two gibel carp (Carassius gibelio) ptpn6 homeologs (Cgptpn6-A and Cgptpn6-B), each of which had three alleles with high identities. Then, relative to Cgptpn6-B, dominant expression in adult tissues and higher upregulated expression of Cgptpn6-A induced by polyinosinic-polycytidylic acid (poly I:C), poly deoxyadenylic-deoxythymidylic (dA:dT) acid and spring viremia of carp virus (SVCV) were uncovered. Finally, we demonstrated that CgSHP1-A (encoded by the Cgptpn6-A gene) and CgSHP1-B (encoded by the Cgptpn6-B gene) act as negative regulators of the RIG-I-like receptor (RLR)-mediated interferon (IFN) response via two mechanisms: the inhibition of CaTBK1-induced phosphorylation of CaMITA shared by CgSHP1-A and CgSHP1-B, and the autophagic degradation of CaMITA exclusively by CgSHP1-A. Meanwhile, the data support that CgSHP1-A and CgSHP1-B have sub-functionalized and that CgSHP1-A overwhelmingly dominates CgSHP1-B in the process of RLR-mediated IFN response. The current study not only sheds light on the regulative mechanism of SHP1 in fish immunity, but also provides a typical case of duplicated gene evolutionary fates.
Keywords
