Frontiers in Cell and Developmental Biology (Jan 2021)
Extracellular Vesicles From Liver Progenitor Cells Downregulates Fibroblast Metabolic Activity and Increase the Expression of Immune-Response Related Molecules
- Felix Royo,
- Felix Royo,
- Mikel Azkargorta,
- Jose L. Lavin,
- Marc Clos-Garcia,
- Ana R. Cortazar,
- Ana R. Cortazar,
- Monika Gonzalez-Lopez,
- Laura Barcena,
- Hernando A. del Portillo,
- Hernando A. del Portillo,
- Hernando A. del Portillo,
- María Yáñez-Mó,
- Antonio Marcilla,
- Antonio Marcilla,
- Francesc E. Borras,
- Francesc E. Borras,
- Francesc E. Borras,
- Hector Peinado,
- Isabel Guerrero,
- Mar Váles-Gómez,
- Unai Cereijo,
- Teresa Sardon,
- Ana M. Aransay,
- Ana M. Aransay,
- Felix Elortza,
- Felix Elortza,
- Juan M. Falcon-Perez,
- Juan M. Falcon-Perez,
- Juan M. Falcon-Perez
Affiliations
- Felix Royo
- Center for Cooperative Research in Biosciences, Bizkaia Technology Park, Bizkaia, Spain
- Felix Royo
- Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas, Madrid, Spain
- Mikel Azkargorta
- Center for Cooperative Research in Biosciences, Bizkaia Technology Park, Bizkaia, Spain
- Jose L. Lavin
- Center for Cooperative Research in Biosciences, Bizkaia Technology Park, Bizkaia, Spain
- Marc Clos-Garcia
- Center for Cooperative Research in Biosciences, Bizkaia Technology Park, Bizkaia, Spain
- Ana R. Cortazar
- Center for Cooperative Research in Biosciences, Bizkaia Technology Park, Bizkaia, Spain
- Ana R. Cortazar
- Centro de Investigación Biomédica en Red de Cáncer, Instituto de Salud Carlos III, Madrid, Spain
- Monika Gonzalez-Lopez
- Center for Cooperative Research in Biosciences, Bizkaia Technology Park, Bizkaia, Spain
- Laura Barcena
- Center for Cooperative Research in Biosciences, Bizkaia Technology Park, Bizkaia, Spain
- Hernando A. del Portillo
- ISGlobal, Hospital Clínic – Universitat de Barcelona, Barcelona, Spain
- Hernando A. del Portillo
- Health Sciences Research Institute Germans Trias i Pujol, Badalona, Spain
- Hernando A. del Portillo
- Institució Catalana de Recerca i Estudis Avançats, Barcelona, Spain
- María Yáñez-Mó
- Departamento de Biología Molecular, Centro de Biología Molecular Severo Ochoa, Instituto de Investigaciones Sanitarias la Princesa, Universidad Autónoma de Madrid, Madrid, Spain
- Antonio Marcilla
- Àrea de Parasitologia, Departament de Farmàcia i Tecnologia Farmacèutica i Parasitologia, Universitat de València, Valencia, Spain
- Antonio Marcilla
- Joint Research Unit on Endocrinology, Nutrition and Clinical Dietetics, Health Research Institute La Fe, Universitat de València, Valencia, Spain
- Francesc E. Borras
- 0Department of Cell Biology, Physiology and Immunology, Autonomous University of Barcelona, Barcelona, Spain
- Francesc E. Borras
- 1REMAR-IVECAT Group-“Germans Trias i Pujol” Health Science Research Institute (IGTP), Badalona, Spain
- Francesc E. Borras
- 2Nephrology Department-“Germans Trias i Pujol” University Hospital, Can Ruti Campus, Badalona, Spain
- Hector Peinado
- 3Microenvironment and Metastasis Laboratory, Molecular Oncology Program, Spanish National Cancer Research Center, Madrid, Spain
- Isabel Guerrero
- 4Tissue and Organ Homeostasis, Centro de Biología Molecular “Severo Ochoa”, Universidad Autónoma de Madrid, Madrid, Spain
- Mar Váles-Gómez
- 5Spanish National Centre for Biotechnology, Spanish National Research Council, Madrid, Spain
- Unai Cereijo
- 6Anaxomics Biotech, Barcelona, Spain
- Teresa Sardon
- 6Anaxomics Biotech, Barcelona, Spain
- Ana M. Aransay
- Center for Cooperative Research in Biosciences, Bizkaia Technology Park, Bizkaia, Spain
- Ana M. Aransay
- Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas, Madrid, Spain
- Felix Elortza
- Center for Cooperative Research in Biosciences, Bizkaia Technology Park, Bizkaia, Spain
- Felix Elortza
- Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas, Madrid, Spain
- Juan M. Falcon-Perez
- Center for Cooperative Research in Biosciences, Bizkaia Technology Park, Bizkaia, Spain
- Juan M. Falcon-Perez
- Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas, Madrid, Spain
- Juan M. Falcon-Perez
- 7Ikerbasque, Basque Foundation for Science, Bilbao, Spain
- DOI
- https://doi.org/10.3389/fcell.2020.613583
- Journal volume & issue
-
Vol. 8
Abstract
Extracellular vesicles (EVs) mediate cell-to-cell crosstalk whose content can induce changes in acceptor cells and their microenvironment. MLP29 cells are mouse liver progenitor cells that release EVs loaded with signaling cues that could affect cell fate. In the current work, we incubated 3T3-L1 mouse fibroblasts with MLP29-derived EVs, and then analyzed changes by proteomics and transcriptomics. Results showed a general downregulation of protein and transcript expression related to proliferative and metabolic routes dependent on TGF-beta. We also observed an increase in the ERBB2 interacting protein (ERBIN) and Cxcl2, together with an induction of ribosome biogenesis and interferon-related response molecules, suggesting the activation of immune system signaling.
Keywords
