Application of Flow Cytometry to Determine Cell DNA Content in the Genetic Breeding of Fish
Xinyan Zhu,
Yang Chen,
Xiaodie Zhang,
Jiaxu Qiang,
Lingtao Nie,
Xinyue Luo,
Binchao Liang,
Kuo Chen,
Fuzhong Yang,
Rurong Zhao,
Chun Zhang
Affiliations
Xinyan Zhu
Engineering Reseaech Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha 410081, China
Yang Chen
Engineering Reseaech Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha 410081, China
Xiaodie Zhang
Engineering Reseaech Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha 410081, China
Jiaxu Qiang
Engineering Reseaech Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha 410081, China
Lingtao Nie
Engineering Reseaech Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha 410081, China
Xinyue Luo
Engineering Reseaech Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha 410081, China
Binchao Liang
Engineering Reseaech Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha 410081, China
Kuo Chen
Engineering Reseaech Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha 410081, China
Fuzhong Yang
College of Agriculture and Forestry Technology, Hunan Applied Technology University, Changde 415000, China
Rurong Zhao
Engineering Reseaech Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha 410081, China
Chun Zhang
Engineering Reseaech Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha 410081, China
In the field of fish genetic breeding, accurately determining the DNA content and ploidy of fish is of great significance. This article introduces the use of flow cytometry (FCM) to measure the DNA content and conduct ploidy analysis by sampling different tissues of freshwater fish species. It describes the FCM detection methods and their effectiveness for different individual tissues. These tissues include embryos and fry, as well as the blood, caudal fins, and sperm of adult live fish, and also specific tissues such as testes, ovaries, gills, spleens, and livers under anatomical conditions. Moreover, the application of ploidy detection to different tissues or individuals in different stages in the practice of fish genetic breeding is analyzed. This research covers samples from different growth stages and a variety of tissue types. The results show that this method exhibits high stability and reliability in the detection of different tissue samples, providing solid data support for subsequent research. It holds significant application value in fish genetic breeding.
