Identification and Verification of <i>qGS11</i>, a QTL Controlling Grain Size and Heading Date in Rice

Abstract

Grain size, shape and weight are important factors influencing grain yield and quality of rice. They are mostly determined by grain length (GL) and grain width (GW). A 13.2 Mb interval, RM167–RM287 on chromosome 11 of rice, was previously found to be associated with variations in 1000-grain weight (TGW). In this study, three populations derived from the indica rice cross Teqing/IRBB52 were used to identify and validate quantitative trait loci (QTLs) controlling GL, GW, TGW and the ratio of GL to GW (RLW) in the RM167–RM287 region. First, two QTL clusters associated with these traits were detected using two populations, segregating the RM167–RM287 interval only. One controlled GL, GW and TGW and was designated as qGS11. The other controlled GL and RLW. The allelic directions of the two QTL clusters on GL were opposite. Then, qGS11 was further mapped in a 1.4 Mb interval using near-isogenic lines, showing a small effect on GL and a relatively large effect on TGW, GW and RLW. Meanwhile, a stable and small effect on the heading date was detected. The allelic direction for the heading date was the opposite for TGW and GW but the same for GL and RLW. The results suggest that qGS11 has the potential for application in rice breeding.

Keywords

• grain length
• grain width
• ratio of grain length to grain width
• grain weight
• quantitative trait locus
• rice (<i>Oryza sativa</i> L.)