AvmM catalyses macrocyclization through dehydration/Michael-type addition in alchivemycin A biosynthesis

Hong Jie Zhu; Bo Zhang; Wanqing Wei; Shuang He Liu; Lang Xiang; Jiapeng Zhu; Rui Hua Jiao; Yasuhiro Igarashi; Ghader Bashiri; Yong Liang; Ren Xiang Tan; Hui Ming Ge

doi:10.1038/s41467-022-32088-4

Nature Communications (Aug 2022)

AvmM catalyses macrocyclization through dehydration/Michael-type addition in alchivemycin A biosynthesis

Hong Jie Zhu,
Bo Zhang,
Wanqing Wei,
Shuang He Liu,
Lang Xiang,
Jiapeng Zhu,
Rui Hua Jiao,
Yasuhiro Igarashi,
Ghader Bashiri,
Yong Liang,
Ren Xiang Tan,
Hui Ming Ge

Affiliations

Hong Jie Zhu: State Key Laboratory of Pharmaceutical Biotechnology, Chemistry and Biomedicine Innovation Centre, Institute of Artificial Intelligence Biomedicine, School of Life Sciences, Nanjing University
Bo Zhang: State Key Laboratory of Pharmaceutical Biotechnology, Chemistry and Biomedicine Innovation Centre, Institute of Artificial Intelligence Biomedicine, School of Life Sciences, Nanjing University
Wanqing Wei: State Key Laboratory of Coordination Chemistry, Jiangsu Key Laboratory of Advanced Organic Materials, Chemistry and Biomedicine Innovation Centre, School of Chemistry and Chemical Engineering, Nanjing University
Shuang He Liu: State Key Laboratory of Pharmaceutical Biotechnology, Chemistry and Biomedicine Innovation Centre, Institute of Artificial Intelligence Biomedicine, School of Life Sciences, Nanjing University
Lang Xiang: State Key Laboratory of Pharmaceutical Biotechnology, Chemistry and Biomedicine Innovation Centre, Institute of Artificial Intelligence Biomedicine, School of Life Sciences, Nanjing University
Jiapeng Zhu: State Key Laboratory Cultivation Base for TCM Quality and Efficacy, School of Medicine and Life Sciences, Nanjing University of Chinese Medicine
Rui Hua Jiao: State Key Laboratory of Pharmaceutical Biotechnology, Chemistry and Biomedicine Innovation Centre, Institute of Artificial Intelligence Biomedicine, School of Life Sciences, Nanjing University
Yasuhiro Igarashi: Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University
Ghader Bashiri: Laboratory of Molecular and Microbial Biochemistry, School of Biological Sciences, The University of Auckland
Yong Liang: State Key Laboratory of Coordination Chemistry, Jiangsu Key Laboratory of Advanced Organic Materials, Chemistry and Biomedicine Innovation Centre, School of Chemistry and Chemical Engineering, Nanjing University
Ren Xiang Tan: State Key Laboratory of Pharmaceutical Biotechnology, Chemistry and Biomedicine Innovation Centre, Institute of Artificial Intelligence Biomedicine, School of Life Sciences, Nanjing University
Hui Ming Ge: State Key Laboratory of Pharmaceutical Biotechnology, Chemistry and Biomedicine Innovation Centre, Institute of Artificial Intelligence Biomedicine, School of Life Sciences, Nanjing University

DOI: https://doi.org/10.1038/s41467-022-32088-4
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 11

Abstract

Read online

Macrocyclization is an important process in bioactive natural product synthesis. Here, the authors report on the study of a macrocyclic ring constructing enzyme in the biosynthesis of alchivemycin A and using gene deletion, biochemical assays and isotope labelling show the enzyme catalyses tandem dehydration and Michael-type addition.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal