Isolation of Microglia from Mouse or Human Tissue

Evan A. Bordt; Carina L. Block; Tiziana Petrozziello; Ghazaleh Sadri-Vakili; Caroline J. Smith; Andrea G. Edlow; Staci D. Bilbo

STAR Protocols (Jun 2020)

Isolation of Microglia from Mouse or Human Tissue

Evan A. Bordt,
Carina L. Block,
Tiziana Petrozziello,
Ghazaleh Sadri-Vakili,
Caroline J. Smith,
Andrea G. Edlow,
Staci D. Bilbo

Affiliations

Evan A. Bordt: Department of Pediatrics, Lurie Center for Autism, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA; Corresponding author
Carina L. Block: Department of Psychology and Neuroscience, Duke University, Durham, NC, USA
Tiziana Petrozziello: Healey Center for ALS at MassGeneral Hospital, NeuroEpigenetics Laboratory, Massachusetts General Hospital, Boston, MA, USA
Ghazaleh Sadri-Vakili: Healey Center for ALS at MassGeneral Hospital, NeuroEpigenetics Laboratory, Massachusetts General Hospital, Boston, MA, USA
Caroline J. Smith: Department of Pediatrics, Lurie Center for Autism, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA; Department of Psychology and Neuroscience, Duke University, Durham, NC, USA
Andrea G. Edlow: Obstetrics, Gynecology, and Reproductive Biology, Harvard Medical School. Massachusetts General Hospital, Vincent Center for Reproductive Biology, Boston, MA, USA
Staci D. Bilbo: Department of Pediatrics, Lurie Center for Autism, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA; Department of Psychology and Neuroscience, Duke University, Durham, NC, USA; Corresponding author

Journal volume & issue: Vol. 1, no. 1
p. 100035

Abstract

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Summary: Microglia are the innate immune cells of the central nervous system. Although numerous methods have been developed to isolate microglia from the brain, the method of dissociation and isolation can have a profound effect on the function of these highly dynamic cells. Here, we present an optimized protocol to isolate CD11b+ cells (microglia) from mouse or human brain tissue using magnetic bead columns. Isolated microglia can be used to model diseases with neuroinflammatory components for potential therapeutic discoveries.For complete details on the use and execution of this protocol, please refer to Hanamsagar et al. (2017), Rivera et al. (2019), and Edlow et al. (2019)

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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