Effects of Beauveria bassiana (Hypocreales) on plant growth and secondary metabolites of extracts of hydroponically cultivated chive (Allium schoenoprasum L. [Amaryllidaceae])

Abstract

The endophytic plant-fungi symbiotic relationship can be explored to improve cultivation of targeted medicinal plant species. The objective of this study was to assess the effects of the cultivation of chive (Allium schoenoprasum) in plant growth medium inoculated with the entomopathogenic fungus, Beauveria bassiana (Hypocreales). Twelve replicates of chive seedlings were exposed to B. bassiana inoculum formulated at concentrations of 0, 1 × 105, 1 × 104 and 1 × 103 conidia mL−1 in a completely randomized design. We assessed plant growth parameters, such as leaf number and plant height weekly and root length, leaf and root fresh and dry weights and secondary metabolites three weeks post-fungal inoculation. The fungus was re-isolated from some of the leaves and roots of the treated plants suggesting that the fungus successfully colonized the plant tissue. Generally, the results indicated that the fungal inoculation had minimal effect on most of the growth parameters assessed in relation to the control. Remarkably, plants exposed to the fungus recorded greater (p < 0.05) total alkaloid, ranging from 2.98 – 3.76 mg atropine equivalent (AE)/g dry weight (DW) compared to the control plants (1.96 mg AE/g DW) for the leaves. This study demonstrated that endophytic fungi could be used to improve the yield of active chemical constituents in cultivated medicinal plants.

Keywords

• Biotechnology
• Microbiology
• Plant biology
• Endophytic fungus
• Chives
• Total alkaloids