Altered miRNA and mRNA Expression in Sika Deer Skeletal Muscle with Age

Boyin Jia; Yuan Liu; Qining Li; Jiali Zhang; Chenxia Ge; Guiwu Wang; Guang Chen; Dongdong Liu; Fuhe Yang

doi:10.3390/genes11020172

Genes (Feb 2020)

Altered miRNA and mRNA Expression in Sika Deer Skeletal Muscle with Age

Boyin Jia,
Yuan Liu,
Qining Li,
Jiali Zhang,
Chenxia Ge,
Guiwu Wang,
Guang Chen,
Dongdong Liu,
Fuhe Yang

Affiliations

Boyin Jia: College of Animal Science and Technology, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China
Yuan Liu: College of Animal Science and Technology, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China
Qining Li: College of Animal Science and Technology, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China
Jiali Zhang: College of Animal Science and Technology, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China
Chenxia Ge: College of Vocational and Technical Education, Changchun Sci-Tech University, 1699 Donghua Street, Changchun 130606, China
Guiwu Wang: Institute of Wild Economic Animals and Plants and State Key Laboratory for Molecular Biology of Special Economical Animals, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun,130112, China
Guang Chen: Key Laboratory of Straw Biology and Utilization, The Ministry of Education, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China
Dongdong Liu: Key Laboratory of Straw Biology and Utilization, The Ministry of Education, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China
Fuhe Yang: Institute of Wild Economic Animals and Plants and State Key Laboratory for Molecular Biology of Special Economical Animals, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun,130112, China

DOI: https://doi.org/10.3390/genes11020172
Journal volume & issue: Vol. 11, no. 2
p. 172

Abstract

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Studies of the gene and miRNA expression profiles associated with the postnatal late growth, development, and aging of skeletal muscle are lacking in sika deer. To understand the molecular mechanisms of the growth and development of sika deer skeletal muscle, we used de novo RNA sequencing (RNA-seq) and microRNA sequencing (miRNA-seq) analyses to determine the differentially expressed (DE) unigenes and miRNAs from skeletal muscle tissues at 1, 3, 5, and 10 years in sika deer. A total of 51,716 unigenes, 171 known miRNAs, and 60 novel miRNAs were identified based on four mRNA and small RNA libraries. A total of 2,044 unigenes and 11 miRNAs were differentially expressed between adolescence and juvenile sika deer, 1,946 unigenes and 4 miRNAs were differentially expressed between adult and adolescent sika deer, and 2,209 unigenes and 1 miRNAs were differentially expressed between aged and adult sika deer. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that DE unigenes and miRNA were mainly related to energy and substance metabolism, processes that are closely associate with the growth, development, and aging of skeletal muscle. We also constructed mRNA−mRNA and miRNA−mRNA interaction networks related to the growth, development, and aging of skeletal muscle. The results show that mRNA (Myh1, Myh2, Myh7, ACTN3, etc.) and miRNAs (miR-133a, miR-133c, miR-192, miR-151-3p, etc.) may play important roles in muscle growth and development, and mRNA (WWP1, DEK, UCP3, FUS, etc.) and miRNAs (miR-17-5p, miR-378b, miR-199a-5p, miR-7, etc.) may have key roles in muscle aging. In this study, we determined the dynamic miRNA and unigenes transcriptome in muscle tissue for the first time in sika deer. The age-dependent miRNAs and unigenes identified will offer insights into the molecular mechanism underlying muscle development, growth, and maintenance and will also provide valuable information for sika deer genetic breeding.

Published in Genes

ISSN: 2073-4425 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General): Genetics
Website: http://www.mdpi.com/journal/genes/

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